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Iodine I 131 Monoclonal Antibody BC8, Fludarabine Phosphate, Total Body Irradiation, and Donor Stem Cell Transplant Followed by Cyclosporine and Mycophenolate Mofetil in Treating Patients With Advanced Acute Myeloid Leukemia or Myelodysplastic Syndrome

A Phase II Trial Combining Radiolabeled BC8 (Anti-CD45) Antibody With Fludarabine and Low Dose TBI Followed by Related or Unrelated PBSC Infusion and Post-Transplant Immunosuppression for Patients With Advanced AML or High Risk Myelodysplastic Syndrome

Status
Terminated
Phases
Phase 2
Study type
Interventional
Source
ClinicalTrials.gov
Registry ID
NCT00119366
Enrollment
18
Registered
2005-07-13
Start date
2003-05-31
Completion date
2019-05-08
Last updated
2022-12-12

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Adult Acute Myeloid Leukemia With 11q23 (MLL) Abnormalities, Adult Acute Myeloid Leukemia With Del(5q), Adult Acute Myeloid Leukemia With Inv(16)(p13;q22), Adult Acute Myeloid Leukemia With t(15;17)(q22;q12), Adult Acute Myeloid Leukemia With t(16;16)(p13;q22), Adult Acute Myeloid Leukemia With t(8;21)(q22;q22), Childhood Myelodysplastic Syndromes, Chronic Myelomonocytic Leukemia, Previously Treated Myelodysplastic Syndromes, Recurrent Adult Acute Myeloid Leukemia, Recurrent Childhood Acute Myeloid Leukemia, Refractory Anemia With Excess Blasts, Refractory Anemia With Excess Blasts in Transformation, Refractory Anemia With Ringed Sideroblasts, Refractory Cytopenia With Multilineage Dysplasia, Secondary Acute Myeloid Leukemia, Secondary Myelodysplastic Syndromes

Brief summary

This phase II trial studies the side effects and best dose of iodine I 131 monoclonal antibody BC8 when given together with fludarabine phosphate, total-body irradiation, and donor stem cell transplant followed by cyclosporine and mycophenolate mofetil in treating patients with acute myeloid leukemia or myelodysplastic syndrome that has spread to other places in the body and usually cannot be cured or controlled with treatment. Giving chemotherapy drugs, such as fludarabine phosphate, and total-body irradiation before a donor peripheral blood stem cell transplant helps stop the growth of cancer or abnormal cells and helps stop the patient's immune system from rejecting the donor's stem cells. Also, radiolabeled monoclonal antibodies, such as iodine I 131 monoclonal antibody BC8, can find cancer cells and carry cancer-killing substances to them without harming normal cells. When the healthy stem cells from a donor are infused into the patient they may help the patient's bone marrow make stem cells, red blood cells, white blood cells, and platelets. Sometimes the transplanted cells from a donor can make an immune response against the body's normal cells. Giving fludarabine phosphate and total-body irradiation before the transplant together with cyclosporine and mycophenolate mofetil after the transplant may stop this from happening. Giving a radiolabeled monoclonal antibody together with donor stem cell transplant, cyclosporine, and mycophenolate mofetil may be an effective treatment for advanced acute myeloid leukemia or myelodysplastic syndromes.

Detailed description

PRIMARY OBJECTIVES: I. To evaluate the maximum tolerated dose (MTD) and the transplant-related mortality (TRM) and toxicity of delivering 131I-BC8 (iodine I 131 monoclonal antibody BC8) (anti-cluster of differentiation \[CD\]45 antibody) at a starting dose of 22 Gy to the normal organ receiving the highest dose in combination with the non-myeloablative regimen of fludarabine (fludarabine phosphate) (FLU), 2 Gy total body irradiation (TBI), cyclosporine (CSP), mycophenolate mofetil (MMF), and human leukocyte antigen (HLA)-matched related or unrelated allogeneic hematopoietic stem cell transplant (HSCT) in patients 16 to 50 years old who have advanced acute myeloid leukemia (AML) or high risk myelodysplastic syndrome (MDS). II. To estimate rates of donor chimerism resulting from this combined preparative regimen and to correlate level of donor chimerism with estimated radiation doses delivered to hematopoietic tissues via antibody. III. To determine rates of disease relapse, graft vs. host disease, and 2-year disease-free survival in patients receiving 131I-BC8 antibody combined with FLU, 2 Gy TBI, CSP, MMF, and HLA-matched related or unrelated allogeneic HSCT. OUTLINE: This is a dose-escalation study of iodine I 131 monoclonal antibody BC8. RADIOIMMUNOTHERAPY: Patients receive therapeutic iodine I 131 monoclonal antibody BC8 intravenously (IV) on day -12. CONDITIONING: Patients receive fludarabine phosphate IV on days -4 to -2 and undergo TBI on day 0. TRANSPLANTATION: After completion of TBI, patients undergo allogeneic peripheral blood stem cell (PBSC) transplant on day 0. IMMUNOSUPPRESSION: Patients with a matched related donor receive cyclosporine IV or orally (PO) twice daily (BID) on days -3 to 56 followed by a taper to day 180 in the absence of graft-versus-host disease. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO BID on days 0 to 27. Patients with a matched unrelated donor receive cyclosporine IV or PO BID on days -3 to 100 followed by a taper to day 180. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO thrice daily (TID) on days 0 to 40 followed by a taper to day 96. After completion of study treatment, patients are followed up at 6, 9, 12, 18, and 24 months and then annually thereafter.

Interventions

DRUGfludarabine phosphate

Given IV

RADIATIONtotal-body irradiation

Undergo TBI

PROCEDUREallogeneic hematopoietic stem cell transplantation

Undergo PBSC transplantation

PROCEDUREperipheral blood stem cell transplantation

Undergo PBSC transplantation

DRUGcyclosporine

Given IV or PO

DRUGmycophenolate mofetil

Given PO

OTHERlaboratory biomarker analysis

Correlative studies

Sponsors

National Cancer Institute (NCI)
CollaboratorNIH
Fred Hutchinson Cancer Center
Lead SponsorOTHER

Study design

Allocation
NON_RANDOMIZED
Intervention model
SEQUENTIAL
Primary purpose
TREATMENT
Masking
NONE

Intervention model description

This study will assess the feasibility and safety of patients with advanced AML or high-risk MDS treated with Iodine-131 BC8 antibody at a starting dose of 22 Gy delivered to the normal organ receiving the highest dose combined with fludarabine and 2 Gy total body irradiation (TBI), plus cyclosporine (CSP)/mycophenolate mofetil (MMF), followed by matched related or unrelated allogeneic hematopoietic stem cell transplantation (HSCT). Determination of the maximum tolerated dose (MTD) will be the major study endpoint. Dose-escalation/de-escalation of radiolabeled BC8 antibody (I-131-BC8) is conducted using a two-stage approach. Under this plan, dose levels during the first stage are increased (or decreased) for individual patients until a dose-limiting toxicity (DLT) is observed, at which point the second stage proceeds with each dose level administered to a cohort of four patients.

Eligibility

Sex/Gender
ALL
Age
16 Years to 50 Years
Healthy volunteers
No

Inclusion criteria

* Patients with advanced AML defined as beyond first remission, primary refractory disease, or evolved from myelodysplastic or myeloproliferative syndromes; or patients with MDS expressed as refractory anemia with excess blasts (RAEB), refractory anemia with excess blasts in transformation (RAEBT), refractory cytopenia with multilineage dysplasia (RCMD), RCMD with ringed sideroblasts (RCMD-RS), or chronic myelomonocytic leukemia (CMML) * Patients not in remission must have CD45-expressing leukemic blasts or myelodysplastic cells; patients in remission do not require phenotyping and may have leukemia previously documented to be CD45 negative (because in remission patients, virtually all antibody binding is to non-malignant cells which make up \>= 95% of nucleated cells in the marrow) * Patients should have a circulating blast count of less than 10,000/mm\^3 (control with hydroxyurea or similar agent is allowed) * Patients must have an estimated creatinine clearance greater than 50/ml per minute (serum creatinine value must be within 28 days prior to registration) * Bilirubin \< 2 times the upper limit of normal * Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) \< 2 times the upper limit of normal * Karnofsky score \>= 70 or Eastern Cooperative Oncology Group (ECOG) =\< 2 * Patients must have an expected survival of \> 60 days and must be free of active infection * Patients must have an HLA-identical sibling donor or an HLA-matched unrelated donor who meets standard Seattle Cancer Care Alliance (SCCA) and/or National Marrow Donor Program (NMDP) or other donor center criteria for peripheral blood stem cell (PBSC) donation; related donors should be matched by molecular methods at the intermediate resolution level at HLA-A, B, C, and developmentally regulated RNA binding protein 1 (DRB1) according to Fred Hutchinson Cancer Research Center (FHCRC) Standard Practice Guidelines and to the allele level at DQB1; unrelated donors should be identified using matching criteria that follows the FHCRC Standard Practice Guidelines limiting the study to eligible donors that are allele matched for HLA-A, B, C, DRB1, and DQB1 (grade 1), and accepting up to one allele mismatch as per Standard Practice grade 2.1 for HLA-A, B, or C * DONOR: Donors must meet HLA matching criteria as well as standard SCCA and/or NMDP or other donor center criteria for PBSC donation

Exclusion criteria

* Circulating antibody against mouse immunoglobulin (human anti-mouse antibody \[HAMA\]) * Prior radiation to maximally tolerated levels to any normal organ * Patients may not have symptomatic coronary artery disease and may not be on cardiac medications for anti-arrhythmic or inotropic effects * Inability to understand or give an informed consent * Patients who are seropositive for human immunodeficiency virus (HIV) * Perceived inability to tolerate diagnostic or therapeutic procedures, particularly treatment in radiation isolation * Patients who have previously undergone autologous or allogeneic HSCT

Design outcomes

Primary

MeasureTime frameDescription
Number of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantUp to 100 days post-transplantThe criteria of Grade III/IV regimen-related toxicity (Bearman) or dose-limiting toxicity (DLT) are as follows: Grade 1 Development of transient chemical abnormalities which are not of major clinical consequence and which reverse without requiring major medical interventions. In general, the intent of this toxicity scale is to observe transient target organ toxicity which is reversible. Grade 2 Development of chemical or laboratory abnormalities that are persistent and which may represent target organ damage that may not be readily reversed. It is anticipated that at this dose of the drug, the toxicity obtained would be manageable by clinical methods but may interfere with other therapies. Grade 3 Development of major clinical, chemical or laboratory abnormalities which represent maximum toxicities without being fatal. This grade of toxicity is designed to be the dose-limiting toxicity.

Secondary

MeasureTime frameDescription
Number of Participants With Transplant Related Mortality Within 100 Days After TransplantUp to 100 days post-transplantNumber of participants that received and completed study treatment who died within 100 days after transplant
Participant Disease Response Within 4 Weeks After Transplant4 weeks after transplantThe number of participants that are in complete remission (CR) or relapsed within 4 weeks after transplant. Complete Remission is defined as complete resolution of all signs of leukemia for at least four weeks with all of the following: * Normal bone marrow with blasts \<5% with normal cellularity, normal megakarypoiesis, more than 15% erythropoiesis and more than 25% granulocytopoiesis. * Normalization of blood counts (no blasts, platelets \>100,000/mm3, granulocytes \>1,500/mm3). * No extramedullary disease. Relapse is measured as follows: * After CR: \>5% blasts in the bone marrow and/or peripheral blood. * Confirmation of relapse by bone marrow analysis with more than 10% blasts. * Extramedullary disease confirmed cytologically or histologically.
Severity of Acute GVHD in Patients Who Completed the Study Treatment100 days after transplantThe severity of acute GVHD is measured based on Graft-vs-Host Disease: Severity of GVHD Grade I +1 to +2 skin rash No gut or liver involvement Grade II +3 skin rash or * 1 gastrointestinal involvement and/or +1 liver involvement Grade III +2 to +4 gastrointestinal involvement and/or * 2 to +4 liver involvement with or without a rash Grade IV Pattern and severity of GVHD similar to grade 3 with extreme constitutional symptoms or death
Number of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 28 and Day 80 after transplantPost-transplant bone marrow samples were collected on day 28 and day 84 after transplant for DNA Chimerism Analysis
Two-year Disease-free Survival of Study Participants Who Completed the Study Regimen2 years post transplantSurvival and complete resolution of all signs of leukemia for 2 years after transplant with all of the following: 1. Normal bone marrow with blasts \<5% with normal cellularity, normal megakarypoiesis, more than 15% erythropoiesis and more than 25% granulocytopoiesis. 2. Normalization of blood counts (no blasts, platelets \>100,000/mm3, granulocytes \>1,500/mm3). 3. No extramedullary disease.

Countries

United States

Participant flow

Recruitment details

Eighteen participants enrolled in the study: 16 - received & completed study treatment 02 - withdrawn from the study

Pre-assignment details

Dose escalation plan: single patients will be entered on the first stage, and escalation by 2 Gy increments in the radiation dose delivered to the normal organ receiving the highest dose will occur until a patient experiences a Grade III/IV regimen-related toxicity (Bearman) or dose-limiting toxicity (DLT), at which point the second stage will begin at the next lower dose level. If the first patient (i.e., at the starting dose level) has DLT, de-escalation will occur by 2 Gy increments

Participants by arm

ArmCount
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal Antibody
RADIOIMMUNOTHERAPY: Patients receive therapeutic iodine I 131 monoclonal antibody BC8 IV on day -12. CONDITIONING: Patients receive fludarabine phosphate IV on days -4 to -2 and undergo TBI on day 0. TRANSPLANTATION: After completion of TBI, patients undergo allogeneic PBSC transplant on day 0. IMMUNOSUPPRESSION: Patients with a matched related donor receive cyclosporine IV or PO BID on days -3 to 56 followed by a taper to day 180 in the absence of graft-versus-host disease. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO 2 BID on days 0 to 27. Patients with a matched unrelated donor receive cyclosporine IV or PO BID on days -3 to 100 followed by a taper to day 180. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO TID on days 0 to 40 followed by a taper to day 96. iodine I 131 monoclonal antibody BC8: Given IV fludarabine phosphate: Given IV total-body irradiation: Undergo TBI allogeneic hematopoietic stem cell transplantation: Undergo PBSC transplantation peripheral blood stem cell transplantation: Undergo PBSC transplantation cyclosporine: Given IV or PO mycophenolate mofetil: Given PO laboratory biomarker analysis: Correlative studies
1
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal Antibody
RADIOIMMUNOTHERAPY: Patients receive therapeutic iodine I 131 monoclonal antibody BC8 IV on day -12. CONDITIONING: Patients receive fludarabine phosphate IV on days -4 to -2 and undergo TBI on day 0. TRANSPLANTATION: After completion of TBI, patients undergo allogeneic PBSC transplant on day 0. IMMUNOSUPPRESSION: Patients with a matched related donor receive cyclosporine IV or PO BID on days -3 to 56 followed by a taper to day 180 in the absence of graft-versus-host disease. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO 2 BID on days 0 to 27. Patients with a matched unrelated donor receive cyclosporine IV or PO BID on days -3 to 100 followed by a taper to day 180. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO TID on days 0 to 40 followed by a taper to day 96. iodine I 131 monoclonal antibody BC8: Given IV fludarabine phosphate: Given IV total-body irradiation: Undergo TBI allogeneic hematopoietic stem cell transplantation: Undergo PBSC transplantation peripheral blood stem cell transplantation: Undergo PBSC transplantation cyclosporine: Given IV or PO mycophenolate mofetil: Given PO laboratory biomarker analysis: Correlative studies
2
Dose Level 8: 24 Gy Iodine-131 + BC8 Monoclonal Antibody
RADIOIMMUNOTHERAPY: Patients receive therapeutic iodine I 131 monoclonal antibody BC8 IV on day -12. CONDITIONING: Patients receive fludarabine phosphate IV on days -4 to -2 and undergo TBI on day 0. TRANSPLANTATION: After completion of TBI, patients undergo allogeneic PBSC transplant on day 0. IMMUNOSUPPRESSION: Patients with a matched related donor receive cyclosporine IV or PO BID on days -3 to 56 followed by a taper to day 180 in the absence of graft-versus-host disease. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO 2 BID on days 0 to 27. Patients with a matched unrelated donor receive cyclosporine IV or PO BID on days -3 to 100 followed by a taper to day 180. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO TID on days 0 to 40 followed by a taper to day 96. iodine I 131 monoclonal antibody BC8: Given IV fludarabine phosphate: Given IV total-body irradiation: Undergo TBI allogeneic hematopoietic stem cell transplantation: Undergo PBSC transplantation peripheral blood stem cell transplantation: Undergo PBSC transplantation cyclosporine: Given IV or PO mycophenolate mofetil: Given PO laboratory biomarker analysis: Correlative studies
3
Dose Level 9: 26 Gy Iodine-131 + BC8 Monoclonal Antibody
RADIOIMMUNOTHERAPY: Patients receive therapeutic iodine I 131 monoclonal antibody BC8 IV on day -12. CONDITIONING: Patients receive fludarabine phosphate IV on days -4 to -2 and undergo TBI on day 0. TRANSPLANTATION: After completion of TBI, patients undergo allogeneic PBSC transplant on day 0. IMMUNOSUPPRESSION: Patients with a matched related donor receive cyclosporine IV or PO BID on days -3 to 56 followed by a taper to day 180 in the absence of graft-versus-host disease. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO 2 BID on days 0 to 27. Patients with a matched unrelated donor receive cyclosporine IV or PO BID on days -3 to 100 followed by a taper to day 180. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO TID on days 0 to 40 followed by a taper to day 96. iodine I 131 monoclonal antibody BC8: Given IV fludarabine phosphate: Given IV total-body irradiation: Undergo TBI allogeneic hematopoietic stem cell transplantation: Undergo PBSC transplantation peripheral blood stem cell transplantation: Undergo PBSC transplantation cyclosporine: Given IV or PO mycophenolate mofetil: Given PO laboratory biomarker analysis: Correlative studies
2
Dose Level 10: 28 Gy Iodine-131 + BC8 Monoclonal Antibody
RADIOIMMUNOTHERAPY: Patients receive therapeutic iodine I 131 monoclonal antibody BC8 IV on day -12. CONDITIONING: Patients receive fludarabine phosphate IV on days -4 to -2 and undergo TBI on day 0. TRANSPLANTATION: After completion of TBI, patients undergo allogeneic PBSC transplant on day 0. IMMUNOSUPPRESSION: Patients with a matched related donor receive cyclosporine IV or PO BID on days -3 to 56 followed by a taper to day 180 in the absence of graft-versus-host disease. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO 2 BID on days 0 to 27. Patients with a matched unrelated donor receive cyclosporine IV or PO BID on days -3 to 100 followed by a taper to day 180. Beginning 4-6 hours after PBSC transplant, these patients also receive mycophenolate mofetil PO TID on days 0 to 40 followed by a taper to day 96. iodine I 131 monoclonal antibody BC8: Given IV fludarabine phosphate: Given IV total-body irradiation: Undergo TBI allogeneic hematopoietic stem cell transplantation: Undergo PBSC transplantation peripheral blood stem cell transplantation: Undergo PBSC transplantation cyclosporine: Given IV or PO mycophenolate mofetil: Given PO laboratory biomarker analysis: Correlative studies
8
Total16

Baseline characteristics

CharacteristicDose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyDose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyDose Level 8: 24 Gy Iodine-131 + BC8 Monoclonal AntibodyDose Level 9: 26 Gy Iodine-131 + BC8 Monoclonal AntibodyDose Level 10: 28 Gy Iodine-131 + BC8 Monoclonal AntibodyTotal
Age, Categorical
<=18 years
0 Participants0 Participants0 Participants0 Participants0 Participants0 Participants
Age, Categorical
>=65 years
0 Participants0 Participants0 Participants0 Participants0 Participants0 Participants
Age, Categorical
Between 18 and 65 years
1 Participants2 Participants3 Participants2 Participants8 Participants16 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
0 Participants0 Participants0 Participants0 Participants0 Participants0 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
1 Participants2 Participants2 Participants2 Participants8 Participants15 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants0 Participants1 Participants0 Participants0 Participants1 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants0 Participants0 Participants0 Participants0 Participants0 Participants
Race (NIH/OMB)
Asian
0 Participants0 Participants0 Participants0 Participants1 Participants1 Participants
Race (NIH/OMB)
Black or African American
0 Participants0 Participants0 Participants0 Participants0 Participants0 Participants
Race (NIH/OMB)
More than one race
0 Participants0 Participants0 Participants0 Participants0 Participants0 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants0 Participants0 Participants0 Participants0 Participants0 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants0 Participants1 Participants0 Participants0 Participants1 Participants
Race (NIH/OMB)
White
1 Participants2 Participants2 Participants2 Participants7 Participants14 Participants
Region of Enrollment
United States
1 participants2 participants3 participants2 participants8 participants16 participants
Sex: Female, Male
Female
1 Participants0 Participants1 Participants1 Participants4 Participants7 Participants
Sex: Female, Male
Male
0 Participants2 Participants2 Participants1 Participants4 Participants9 Participants

Adverse events

Event typeEG000
affected / at risk
EG001
affected / at risk
EG002
affected / at risk
EG003
affected / at risk
EG004
affected / at risk
deaths
Total, all-cause mortality
1 / 10 / 22 / 32 / 22 / 8
other
Total, other adverse events
0 / 10 / 21 / 32 / 25 / 8
serious
Total, serious adverse events
1 / 12 / 23 / 32 / 28 / 8

Outcome results

Primary

Number of Participants With Dose-limiting Toxicities (DLT) 100 Days After Transplant

The criteria of Grade III/IV regimen-related toxicity (Bearman) or dose-limiting toxicity (DLT) are as follows: Grade 1 Development of transient chemical abnormalities which are not of major clinical consequence and which reverse without requiring major medical interventions. In general, the intent of this toxicity scale is to observe transient target organ toxicity which is reversible. Grade 2 Development of chemical or laboratory abnormalities that are persistent and which may represent target organ damage that may not be readily reversed. It is anticipated that at this dose of the drug, the toxicity obtained would be manageable by clinical methods but may interfere with other therapies. Grade 3 Development of major clinical, chemical or laboratory abnormalities which represent maximum toxicities without being fatal. This grade of toxicity is designed to be the dose-limiting toxicity.

Time frame: Up to 100 days post-transplant

Population: Study participants that received and completed study treatment

ArmMeasureCategoryValue (COUNT_OF_PARTICIPANTS)
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with no dose-limiting toxicities 100 days after transplant1 Participants
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with dose-limiting toxicities 100 days after transplant0 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with no dose-limiting toxicities 100 days after transplant2 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with dose-limiting toxicities 100 days after transplant0 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Number of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with no dose-limiting toxicities 100 days after transplant3 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Number of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with dose-limiting toxicities 100 days after transplant0 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Number of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with dose-limiting toxicities 100 days after transplant0 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Number of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with no dose-limiting toxicities 100 days after transplant2 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Number of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with no dose-limiting toxicities 100 days after transplant8 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Number of Participants With Dose-limiting Toxicities (DLT) 100 Days After TransplantNumber of participants with dose-limiting toxicities 100 days after transplant0 Participants
Secondary

Number of Participants With 100% Donor Chimerism at Day 28 and Day 84

Post-transplant bone marrow samples were collected on day 28 and day 84 after transplant for DNA Chimerism Analysis

Time frame: Day 28 and Day 80 after transplant

Population: Patients that completed the study regimen

ArmMeasureGroupValue (COUNT_OF_PARTICIPANTS)
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 28 Donor Chimerism0 Participants
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 84 Donor Chimerism0 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 28 Donor Chimerism1 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 84 Donor Chimerism1 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Number of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 28 Donor Chimerism3 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Number of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 84 Donor Chimerism3 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Number of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 84 Donor Chimerism2 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Number of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 28 Donor Chimerism2 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Number of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 28 Donor Chimerism7 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Number of Participants With 100% Donor Chimerism at Day 28 and Day 84Day 84 Donor Chimerism4 Participants
Secondary

Number of Participants With Transplant Related Mortality Within 100 Days After Transplant

Number of participants that received and completed study treatment who died within 100 days after transplant

Time frame: Up to 100 days post-transplant

Population: Participants that received and completed study treatment who died within 100 days after transplant

ArmMeasureCategoryValue (COUNT_OF_PARTICIPANTS)
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who died within 100 days after transplant1 Participants
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who are alive > 100 days after transplant0 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who died within 100 days after transplant0 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyNumber of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who are alive > 100 days after transplant2 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Number of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who died within 100 days after transplant0 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Number of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who are alive > 100 days after transplant3 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Number of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who are alive > 100 days after transplant2 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Number of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who died within 100 days after transplant0 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Number of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who died within 100 days after transplant1 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Number of Participants With Transplant Related Mortality Within 100 Days After TransplantNumber of participants who are alive > 100 days after transplant7 Participants
Secondary

Participant Disease Response Within 4 Weeks After Transplant

The number of participants that are in complete remission (CR) or relapsed within 4 weeks after transplant. Complete Remission is defined as complete resolution of all signs of leukemia for at least four weeks with all of the following: * Normal bone marrow with blasts \<5% with normal cellularity, normal megakarypoiesis, more than 15% erythropoiesis and more than 25% granulocytopoiesis. * Normalization of blood counts (no blasts, platelets \>100,000/mm3, granulocytes \>1,500/mm3). * No extramedullary disease. Relapse is measured as follows: * After CR: \>5% blasts in the bone marrow and/or peripheral blood. * Confirmation of relapse by bone marrow analysis with more than 10% blasts. * Extramedullary disease confirmed cytologically or histologically.

Time frame: 4 weeks after transplant

Population: Participants that received and completed study treatment

ArmMeasureGroupValue (COUNT_OF_PARTICIPANTS)
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyParticipant Disease Response Within 4 Weeks After TransplantNumber of participants that are in CR 4 weeks after transplant0 Participants
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyParticipant Disease Response Within 4 Weeks After TransplantNumber of participants that relapsed 4 weeks after transplant1 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyParticipant Disease Response Within 4 Weeks After TransplantNumber of participants that are in CR 4 weeks after transplant2 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyParticipant Disease Response Within 4 Weeks After TransplantNumber of participants that relapsed 4 weeks after transplant0 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Participant Disease Response Within 4 Weeks After TransplantNumber of participants that are in CR 4 weeks after transplant3 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Participant Disease Response Within 4 Weeks After TransplantNumber of participants that relapsed 4 weeks after transplant0 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Participant Disease Response Within 4 Weeks After TransplantNumber of participants that relapsed 4 weeks after transplant1 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Participant Disease Response Within 4 Weeks After TransplantNumber of participants that are in CR 4 weeks after transplant1 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Participant Disease Response Within 4 Weeks After TransplantNumber of participants that are in CR 4 weeks after transplant6 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Participant Disease Response Within 4 Weeks After TransplantNumber of participants that relapsed 4 weeks after transplant2 Participants
Secondary

Severity of Acute GVHD in Patients Who Completed the Study Treatment

The severity of acute GVHD is measured based on Graft-vs-Host Disease: Severity of GVHD Grade I +1 to +2 skin rash No gut or liver involvement Grade II +3 skin rash or * 1 gastrointestinal involvement and/or +1 liver involvement Grade III +2 to +4 gastrointestinal involvement and/or * 2 to +4 liver involvement with or without a rash Grade IV Pattern and severity of GVHD similar to grade 3 with extreme constitutional symptoms or death

Time frame: 100 days after transplant

Population: Study participants that completed study treatment

ArmMeasureCategoryValue (COUNT_OF_PARTICIPANTS)
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodySeverity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 0-1 GVHD0 Participants
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodySeverity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 3 GVHD0 Participants
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodySeverity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 2 GVHD1 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodySeverity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 2 GVHD2 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodySeverity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 0-1 GVHD0 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodySeverity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 3 GVHD0 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Severity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 2 GVHD3 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Severity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 0-1 GVHD0 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Severity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 3 GVHD0 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Severity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 0-1 GVHD0 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Severity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 3 GVHD0 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Severity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 2 GVHD2 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Severity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 2 GVHD4 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Severity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 0-1 GVHD3 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Severity of Acute GVHD in Patients Who Completed the Study TreatmentNumber of participants with Grade 3 GVHD1 Participants
Secondary

Two-year Disease-free Survival of Study Participants Who Completed the Study Regimen

Survival and complete resolution of all signs of leukemia for 2 years after transplant with all of the following: 1. Normal bone marrow with blasts \<5% with normal cellularity, normal megakarypoiesis, more than 15% erythropoiesis and more than 25% granulocytopoiesis. 2. Normalization of blood counts (no blasts, platelets \>100,000/mm3, granulocytes \>1,500/mm3). 3. No extramedullary disease.

Time frame: 2 years post transplant

Population: Participants who are alive and disease-free 2 years after transplant

ArmMeasureValue (COUNT_OF_PARTICIPANTS)
Dose Level 1: 12 Gy Iodine-131 + BC8 Monoclonal AntibodyTwo-year Disease-free Survival of Study Participants Who Completed the Study Regimen0 Participants
Dose Level 7: 22 Gy Iodine-131 + BC8 Monoclonal AntibodyTwo-year Disease-free Survival of Study Participants Who Completed the Study Regimen2 Participants
Dose Level 8: 24 Gy Iodine-131 + BC8Two-year Disease-free Survival of Study Participants Who Completed the Study Regimen1 Participants
Dose Level 9: 26 Gy Iodine-131 + BC8Two-year Disease-free Survival of Study Participants Who Completed the Study Regimen0 Participants
Dose Level 10: 28 Gy Iodine-131 + BC8Two-year Disease-free Survival of Study Participants Who Completed the Study Regimen2 Participants

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026