Asthma, Healthy
Conditions
Keywords
Adenosine receptors, asthma, sputum, peripheral blood, healthy
Brief summary
The purpose of this study is to determine whether asthmatics have different adenosine receptor expression profiles than healthy controls. We hypothesize that asthmatics will have increased adenosine receptor expression versus control subjects. We also want to study the effects of allergen inhalation on adenosine receptor expression in asthmatics. We believe that adenosine receptor expression will be upregulated after allergen inhalation. Both hypotheses are being tested in sputum and peripheral blood.
Interventions
Sponsors
Groningen Research Institute for Asthma and COPD
Study design
Observational model
DEFINED_POPULATION
Time perspective
OTHER
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
Asthmatic Subjects - Inclusion Criteria: * Clinical diagnosis of asthma * Steroid naive or subjects not using their inhaled steroids for the last 4 weeks, short-acting beta-agonist for rescue medication * FEV1 \> 70% of predicted * Positive skin prick test for house dust mite, cat or grass pollen * PC20 methacholine or histamine \< 8 mg/ml Asthmatic Subjects -
Exclusion criteria
* Use of systemic steroids in the previous 6 weeks * Asthma exacerbations in the previous 6 weeks * Current or ex-smokers with ≥ 10 pack-years (≥ 2 pipe pack-years), ex smokers who stopped less than 1 year ago Healthy Subjects - Inclusion Criteria: * No airway complaints * FEV1 \> 90% of predicted * Negative skin prick test * PC20 methacholine or histamine \> 8 mg/ml Healthy Subjects -
Countries
Netherlands
Outcome results
None listed