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MDX-010 in Treating Patients With Recurrent or Refractory Lymphoma

Phase I/II Study of Anti-CTLA-4 Monoclonal Antibody (MDX-010) in B-cell Non-Hodgkin's Lymphoma

Status
Terminated
Phases
Phase 1Phase 2
Study type
Interventional
Source
ClinicalTrials.gov
Registry ID
NCT00089076
Enrollment
18
Registered
2004-08-05
Start date
2004-06-30
Completion date
2009-10-31
Last updated
2014-05-30

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Adult Grade III Lymphomatoid Granulomatosis, B-cell Chronic Lymphocytic Leukemia, Cutaneous B-cell Non-Hodgkin Lymphoma, Extranodal Marginal Zone B-cell Lymphoma of Mucosa-associated Lymphoid Tissue, Intraocular Lymphoma, Nodal Marginal Zone B-cell Lymphoma, Recurrent Adult Burkitt Lymphoma, Recurrent Adult Diffuse Large Cell Lymphoma, Recurrent Adult Diffuse Mixed Cell Lymphoma, Recurrent Adult Diffuse Small Cleaved Cell Lymphoma, Recurrent Adult Grade III Lymphomatoid Granulomatosis, Recurrent Adult Hodgkin Lymphoma, Recurrent Adult Immunoblastic Large Cell Lymphoma, Recurrent Adult Lymphoblastic Lymphoma, Recurrent Grade 1 Follicular Lymphoma, Recurrent Grade 2 Follicular Lymphoma, Recurrent Mantle Cell Lymphoma, Recurrent Marginal Zone Lymphoma, Refractory Hairy Cell Leukemia, Small Intestine Lymphoma, Splenic Marginal Zone Lymphoma, Testicular Lymphoma, Waldenström Macroglobulinemia

Brief summary

Biological therapies, such as MDX-010, work in different ways to stimulate the immune system and stop cancer cells from growing. This phase I/II trial is studying the side effects and best dose of MDX-010 and to see how well it works in treating patients with recurrent or refractory B-cell non-Hodgkin's lymphoma.

Detailed description

PRIMARY OBJECTIVES: I. To characterize the safety profile of MDX-010 (ipilimumab) monoclonal antibody and identify a tolerable immunologically active dose level in B cell lymphoma patients. II. To evaluate the clinical response rate in B cell lymphoma patients treated with MDX-010. SECONDARY OBJECTIVES: I. To evaluate the phenotype and function of memory T cells before and after treatment with MDX-010 by: * Quantitation and phenotypic characterization of peripheral blood and tumor infiltrating T-cells, including cluster of differentiation (CD)4+CD25+ regulatory T cells. * Measurement of tumor-specific T cells in peripheral blood lymphocytes. * Measuring proliferation of memory T cells in response to recall antigens (tetanus toxoid and keyhole limpet hemocyanin \[KLH\]). II. Measurement of anti-tumor antibodies in serum pre- and post-therapy. III. To evaluate the time to progression. IV. To evaluate the duration of response to treatment with MDX-010. OUTLINE: This is a multicenter, open-label, phase I, dose-escalation study followed by a phase II study. Patients are grouped according to prior treatment with a vaccine therapy for lymphoma (yes vs no). PHASE I: Patients receive anti-cytotoxic T-lymphocyte-associated antigen-4 monoclonal antibody (MDX-010) IV over 90 minutes on day 1. Treatment repeats every 28 days for a total of 4 courses in the absence of disease progression or unacceptable toxicity. Cohorts of 6 patients from each group receive escalating doses of MDX-010 until the maximum tolerated dose (MTD) is determined. The MTD is defined as the dose preceding that at which 2 of 6 patients experience dose-limiting toxicity. PHASE II: Patients receive MDX-010 as in phase I at the MTD. Patients are followed at 1 and 4 months and then every 6 months for up to 2 years.

Interventions

BIOLOGICALipilimumab

Given IV

OTHERlaboratory biomarker analysis

Correlative studies

Sponsors

National Cancer Institute (NCI)
Lead SponsorNIH

Study design

Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE

Eligibility

Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No

Inclusion criteria

* Histologic proof of recurring or residual follicular B-cell non-Hodgkin's lymphoma (grade I or II), by Revised European American Lymphoma Classification (REAL) or World Health Organization (WHO) classifications which has relapsed or persisted after 3 or fewer conventional therapies, including chemotherapy or monoclonal antibody therapy; note: all patients with previously treated B-cell lymphomas of any histology with the exception of small lymphocytic lymphoma/chronic lymphocytic leukemia (CLL) are eligible * Tumor measurable by computed tomography (CT) scans (at least one pathologic node measuring 2.0 x 2.0 cm, or 2 nodes measuring \> 1.5 x 1.5 cm after collection of tumor for immunologic analyses) * At least one prior treatment regimen but no more than 3 prior chemotherapy regimens; patients previously treated with monoclonal antibodies or radiotherapy to a single site will be eligible; these therapies will be considered prior treatment regimens but will not be considered as prior chemotherapy; tumor vaccines will not be counted as prior therapies, as all such agents are investigational * Absolute neutrophil count (ANC) \>= 1000/uL * Platelets (PLT) \>= 75,000/uL * Total bilirubin =\< 1.5 x upper limit of normal (ULN) * Aspartate aminotransferase (AST) =\< 3 x upper limit or normal (ULN) * Creatinine =\< 1.5 x ULN * Hemoglobin \>= 8 g/dL * Ability to provide informed consent * Willingness to return to the Mayo Clinic Rochester or the University of California, Los Angeles for follow up * Life expectancy \>= 24 weeks * Willingness to provide all biologic specimens as required by the protocol

Exclusion criteria

* Known standard therapy for the patient's disease that is potentially curative or definitely capable of extending life expectancy * Eastern Cooperative Oncology Group (ECOG) performance status (PS) 2, 3, or 4 * Any uncontrolled infection, hepatitis C virus (HCV)+ (unless HCV ribonucleic acid \[RNA\]-negative by polymerase chain reaction \[PCR\]) or hepatitis B surface antigen (HBsAg)+, or human immunodeficiency virus (HIV) positive patients or patients with known immune deficiency states * Previous MDX-010 therapy regardless of interval since last treatment * Prior treatment with fludarabine or 2-chlorodeoxyadenosine =\< 12 months prior to registration * Failure to fully recover from acute, reversible effects of prior chemotherapy regardless of interval since last treatment * New York Heart Association classification III or IV or a history of angina pectoris requiring active treatment * Clinical evidence of central nervous system involvement by lymphoma * Any of the following: * Pregnant women * Nursing women * Men or women of childbearing potential who are unwilling to employ adequate contraception (condoms, diaphragm, birth control pills, injections, intrauterine device \[IUD\], or abstinence, etc.) * Other concurrent chemotherapy, immunotherapy, radiotherapy, or any ancillary therapy considered investigational (utilized for a non-Food and Drug Administration \[FDA\]-approved indication and in the context of a research investigation) * Diagnosis of small lymphocytic lymphoma/chronic lymphocytic leukemia (CLL) * Any requirement for concurrent steroid therapy, including use of inhaled steroids for asthma * History of autoimmune disease requiring systemic therapy with immunosuppressive drugs, including but not limited to rheumatoid arthritis, inflammatory bowel disease, systemic lupus erythematosus, multiple sclerosis, or psoriasis * Antinuclear antibody (ANA) titer or rheumatoid factor titer \> 3x institutional ULN

Design outcomes

Primary

MeasureTime frameDescription
Number of Overall Confirmed Responses(Complete Response or Partial Response)From registration to month 7Confirmed response is at least a 50% decrease in the sum of the products of the greatest diameters (SPD) of the six largest dominant nodes or nodal masses and no increase in the size of other nodes, liver, or spleen and splenic and hepatic nodules must regress by at least 50% in the SPD and no new sites of disease.

Secondary

MeasureTime frameDescription
Overall Survival (Phase 2)From registration to death (up to 2 years)The overall survival or survival time is defined as the time from registration to death due to any cause. The distribution of overall survival will be estimated using the method of Kaplan-Meier.
Duration of Response (Phase 2)From response to progression (up to 2 years)Duration of response will be calculated from the documentation of confirmed response until the date of progression in the subset of patients who respond.
Mean Change in % of CD3+CD4+ for Marker HLA-DR+Before treatment to 1 month after therapy initiationFlow cytometric analysis of T-cell surface markers before and 1 month after initiation therapy
Time to Progression (Phase 2)From registration to progression (up to 2 years)The time to progression is defined as the time from registration to the time of progression. Those who die will be considered to have had disease progression unless documented evidence clearly indicates no progression has occurred. The distribution of time to progression will be estimated using the method of Kaplan-Meier.
Mean Change in % of CD3+CD4+ for Marker CD45RO+Before treatment to 1 month after therapy initiationFlow cytometric analysis of T-cell surface markers before and 1 month after initiation therapy
Mean Change in % of CD3+CD4- for the Marker CD45RO+Before treatment to 1 month after therapy initiationFlow cytometric analysis of T-cell surface markers before and 1 month after initiation therapy
Mean Change in % of CD3+CD4- for Marker HLA-DR+Before treatment to 1 month after therapy initiationFlow cytometric analysis of T-cell surface markers before and 1 month after initiation therapy

Countries

United States

Participant flow

Recruitment details

Participants were recruited from 2 medical clinics in the United States between August \> 2004 and September 2007.

Pre-assignment details

This was a phase I/II trial. A total of 18 participants were accrued, all to the phase I portion. This trial was terminated due to study design during the phase I; therefore, the phase II portion will never open. No results from the phase II portion are available.

Participants by arm

ArmCount
MDX-010
Patients receive anti-cytotoxic T-lymphocyte-associated antigen-4 monoclonal antibody (MDX-010) IV over 90 minutes on day 1. Treatment repeats every 28 days for a total of 4 courses in the absence of disease progression or unacceptable toxicity.
18
Total18

Withdrawals & dropouts

PeriodReasonFG000
Overall StudyAlternate Therapy1
Overall StudyProgression7
Overall StudyWithdrawal by Subject2

Baseline characteristics

CharacteristicMDX-010
Age, Continuous56 years
Disease Histology
Diffuse large B-cell lymphoma
3 participants
Disease Histology
Follicular grade 1 lymphoma
9 participants
Disease Histology
Follicular grade 2 lymphoma
5 participants
Disease Histology
Mantle cell lymphoma
1 participants
Dose Level
3 mg/kg first dose, then 1 mg/kg monthly x 3 doses
12 participants
Dose Level
3 mg/kg monthly x 4 doses
6 participants
Number of prior treatments2 Treatments
Region of Enrollment
United States
18 participants
Sex: Female, Male
Female
6 Participants
Sex: Female, Male
Male
12 Participants

Adverse events

Event typeEG000
affected / at risk
deaths
Total, all-cause mortality
— / —
other
Total, other adverse events
16 / 18
serious
Total, serious adverse events
8 / 18

Outcome results

Primary

Number of Overall Confirmed Responses(Complete Response or Partial Response)

Confirmed response is at least a 50% decrease in the sum of the products of the greatest diameters (SPD) of the six largest dominant nodes or nodal masses and no increase in the size of other nodes, liver, or spleen and splenic and hepatic nodules must regress by at least 50% in the SPD and no new sites of disease.

Time frame: From registration to month 7

ArmMeasureValue (NUMBER)
MDX-010Number of Overall Confirmed Responses(Complete Response or Partial Response)2 participants
Secondary

Duration of Response (Phase 2)

Duration of response will be calculated from the documentation of confirmed response until the date of progression in the subset of patients who respond.

Time frame: From response to progression (up to 2 years)

Population: No participants proceeded to Phase 2 for evaluation.

Secondary

Mean Change in % of CD3+CD4+ for Marker CD45RO+

Flow cytometric analysis of T-cell surface markers before and 1 month after initiation therapy

Time frame: Before treatment to 1 month after therapy initiation

Population: The analysis population contains patients that had peripheral blood available for analysis from before and 1 month after initiating therapy along with being able to conduct the analysis on the marker. This resulted in the number of participants analyzed being less than the enrolled participants.

ArmMeasureValue (MEAN)Dispersion
MDX-010Mean Change in % of CD3+CD4+ for Marker CD45RO+5.0 percentage of change of CD3+CD4+Standard Error 1.5
Secondary

Mean Change in % of CD3+CD4- for Marker HLA-DR+

Flow cytometric analysis of T-cell surface markers before and 1 month after initiation therapy

Time frame: Before treatment to 1 month after therapy initiation

Population: The analysis population contains patients that had peripheral blood available for analysis from before and 1 month after initiating therapy along with being able to conduct the analysis on the marker. This resulted in the number of participants analyzed being less than the enrolled participants.

ArmMeasureValue (MEAN)Dispersion
MDX-010Mean Change in % of CD3+CD4- for Marker HLA-DR+1.9 percentage of change of CD3+CD4-Standard Error 2
Secondary

Mean Change in % of CD3+CD4+ for Marker HLA-DR+

Flow cytometric analysis of T-cell surface markers before and 1 month after initiation therapy

Time frame: Before treatment to 1 month after therapy initiation

Population: The analysis population contains patients that had peripheral blood available for analysis from before and 1 month after initiating therapy along with being able to conduct the analysis on the marker. This resulted in the number of participants analyzed being less than the enrolled participants.

ArmMeasureValue (MEAN)Dispersion
MDX-010Mean Change in % of CD3+CD4+ for Marker HLA-DR+1.8 percentage of change of CD3+CD4+Standard Error 1.2
Secondary

Mean Change in % of CD3+CD4- for the Marker CD45RO+

Flow cytometric analysis of T-cell surface markers before and 1 month after initiation therapy

Time frame: Before treatment to 1 month after therapy initiation

Population: The analysis population contains patients that had peripheral blood available for analysis from before and 1 month after initiating therapy along with being able to conduct the analysis on the marker. This resulted in the number of participants analyzed being less than the enrolled participants.

ArmMeasureValue (MEAN)Dispersion
MDX-010Mean Change in % of CD3+CD4- for the Marker CD45RO+2.8 percentage of change of CD3+CD4-Standard Error 0.9
Secondary

Overall Survival (Phase 2)

The overall survival or survival time is defined as the time from registration to death due to any cause. The distribution of overall survival will be estimated using the method of Kaplan-Meier.

Time frame: From registration to death (up to 2 years)

Population: No participants proceeded to Phase 2 for evaluation.

Secondary

Time to Progression (Phase 2)

The time to progression is defined as the time from registration to the time of progression. Those who die will be considered to have had disease progression unless documented evidence clearly indicates no progression has occurred. The distribution of time to progression will be estimated using the method of Kaplan-Meier.

Time frame: From registration to progression (up to 2 years)

Population: No participants proceeded to Phase 2 for evaluation.

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026