Breast Cancer
Conditions
Keywords
stage II breast cancer, stage IIIA breast cancer, stage IIIB breast cancer, stage IIIC breast cancer, stage IA breast cancer, stage IB breast cancer
Brief summary
RATIONALE: Diagnostic procedures, such as nipple aspiration, ductal lavage, and breast duct endoscopy, may help doctors detect and assess breast cancer cells early and plan more effective treatment. PURPOSE: This phase II trial is studying how well nipple aspiration, ductal lavage, and duct endoscopy work in assessing cancer cells in women who are undergoing surgery for breast cancer.
Detailed description
OBJECTIVES: Primary * Correlate the cell yield and morphology findings from ductal lavage with duct endoscopy findings and surgical pathology findings in women with breast cancer. * Determine the sensitivity and specificity of nipple aspiration, ductal lavage, and duct endoscopy in detecting established breast cancer in these patients. Secondary * Compare the intraduct environment of cancer-involved ducts in the affected breast vs the ductal systems in the contralateral breast of these patients. * Perform immunohistochemical analysis (including estrogen receptor, progesterone receptor, HER2-neu receptor, epidermal growth factor receptor, p53, and proliferation marker expression) for markers potentially associated with breast cancer in these patients. * Determine potential molecular markers of malignancy by gene methylation, gene expression, and proteomics in these patients. * Compare the biochemistry and protein analysis of the intraductal fluid vs serum analysis in these patients. OUTLINE: Patients undergo nipple aspiration, ductal lavage, and duct endoscopy under general anesthesia immediately before breast surgery. Fluid and tissue obtained are examined for tumor markers by immunohistochemistry. Candidate genes are analyzed by gene methylation, gene expression arrays, and proteomic profiling. Patients are followed at 24 hours and at 1 week. PROJECTED ACCRUAL: A total of 100 patients will be accrued for this study within 1 year.
Interventions
GENETICcytogenetic analysis
GENETICproteomic profiling
OTHERcytology specimen collection procedure
OTHERimmunohistochemistry staining method
OTHERlaboratory biomarker analysis
PROCEDUREbreast duct lavage
Sponsors
Royal Marsden NHS Foundation Trust
Study design
Primary purpose
DIAGNOSTIC
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
DISEASE CHARACTERISTICS: * Diagnosis of breast cancer * No metastatic disease * No inflammatory breast cancer * Hormone receptor status: * Not specified PATIENT CHARACTERISTICS: Age * 18 and over Sex * Female Menopausal status * Not specified Performance status * Not specified Life expectancy * Not specified Hematopoietic * Not specified Hepatic * Not specified Renal * Not specified Other * No active infection or inflammation in the breast under study * No known allergy to eutectic mixture of local anesthetics (EMLA®) cream or lidocaine * No severe illness * Not unconscious * No mental illness or handicap * No nursing within the past 12 months * Not pregnant PRIOR CONCURRENT THERAPY: Biologic therapy * Not specified Chemotherapy * Not specified Endocrine therapy * Not specified Radiotherapy * Not specified Surgery * No prior breast implantation on side of proposed lavage * No prior subareolar (e.g., papilloma resections, biopsies, or fine needle aspirations) or other surgery that may disrupt the ductal systems within 2 cm of the nipple * Biopsies and fine-needle aspirations \> 2 cm from the nipple are allowed
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Correlation of cell yields and morphology in ductal lavage with duct endoscopy appearances and findings | — |
| Sensitivity and specificity of ductal lavage with or without ductoscopy in detecting atypical cells | — |
| Comparison of cell yields vs final surgical pathology of the operative specimens | — |
Countries
United Kingdom
Outcome results
None listed