AIH DIHS iDILI Healthy Control Autoimmune hepatitis, Drug-induced hypersensitivity syndrome, Idiosyncratic drug-induced liver injury, Organoid
Conditions
Interventions
None listed
Sponsors
Takebe Takanori
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Patients diagnosed with either Autoimmune Hepatitis (AIH), Drug-induced Hypersensitivity Syndrome (DIHS), or Idiosyncratic Drug-induced Liver Injury (iDILI). Healthy subjects appropriate as controls for this study. Individuals who have provided written informed consent to participate in this study.
Exclusion criteria
Exclusion criteria: Individuals deemed unsuitable for this study by the principal investigator or co-investigator for medical or other reasons.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| (AIH) Identification of candidate self-antigen peptides presented in an HLA-DR4-dependent manner. (AIH) Identification of autoreactive T-cell clones (TCR sequences) that respond to HLO co-culture in an HLA-DR4-dependent manner. (DIHS/iDILI) Identification of potential HLA haplotypes and associated TCR repertoires involved in drug-specific responses using an organoid model/screening system. | — |
Secondary
| Measure | Time frame |
|---|---|
| (AIH) Degree of HLA-DR4-dependent cytotoxicity and cytokine production profiles. (DIHS/iDILI) Degree of drug-dependent cytotoxicity, cytokine production profiles, and T-cell proliferation responses. Correlation analysis of TCR repertoires identified in the iPS model and those in actual patient samples. Validation of T-cell responses to identified candidate self-antigen peptides. | — |
Contacts
Public ContactMasashi Okamoto
Graduate School of Medicine, The University of Osaka
Outcome results
None listed