DGC Residual Effects Study

Single institution study to evaluate the effect of residual solution and additional washings after density gradient centrifugation treatment on sperm findings

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

JPRN

Registry ID

JPRN-jRCT1040230048

Enrollment

Registered

2023-06-24

Start date

2023-06-24

Completion date

Unknown

Last updated

2026-06-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

male infertile patient

Interventions

None listed

Eligibility

Sex/Gender

Male

Inclusion criteria

Inclusion criteria: 1) Male patients over 18 years old 2) Patients whose background can be confirmed in the medical records 3) Patients with a total sperm concentration of 2.0 x 10^6 /ml or higher and a motility rate of 40% or higher in the original semen 4) Patients who have given written consent to participate in the study.

Exclusion criteria

Exclusion criteria: 1) Those with severe oligozoospermia, severe spermatasthenia, severe hypospermia or azoospermia 2) Patients who are deemed inappropriate for inclusion in the study by the principal investigator or research coordinator.

Design outcomes

Primary

Measure	Time frame
Semen findings before DGC treatment (fluid volume, total sperm concentration, motility rate, malformation rate, and SDF rate) and immediately after DGC treatment and after 4 hours of incubation (semen volume, total sperm concentration, motility rate, malformation rate, and SDF rate)	—

Secondary

Measure	Time frame
The residual solution after DGC treatment and additional washings were performed and the effects on semen findings and SDF immediately after treatment and after 4 hours of incubation were evaluated using detailed parameters obtained during general semen/sperm tests (pH, LIN (linearity), STR (linearity), WOB (curvature), VAP (mean velocity), VSL (velocity of curvature), VCL (velocity of straight line), ALH (head amplitude value), BCF (head amplitude frequency), Head Length (length of sperm head), Head Width (width of sperm head), Head Length (length of sperm head), Head Length (length of sperm head), Head Width (length of sperm head), and Head Length (width of sperm head)). ), ALH (head amplitude value), BCF (head amplitude frequency), Head Length (length of sperm head), Head Width (width of sperm head), Head Perimeter (circumference of sperm head), Head Area (area of sperm head), Tail Length	—

Measure

Time frame

The residual solution after DGC treatment and additional washings were performed and the effects on semen findings and SDF immediately after treatment and after 4 hours of incubation were evaluated using detailed parameters obtained during general semen/sperm tests (pH, LIN (linearity), STR (linearity), WOB (curvature), VAP (mean velocity), VSL (velocity of curvature), VCL (velocity of straight line), ALH (head amplitude value), BCF (head amplitude frequency), Head Length (length of sperm head), Head Width (width of sperm head), Head Length (length of sperm head), Head Length (length of sperm head), Head Width (length of sperm head), and Head Length (width of sperm head)). ), ALH (head amplitude value), BCF (head amplitude frequency), Head Length (length of sperm head), Head Width (width of sperm head), Head Perimeter (circumference of sperm head), Head Area (area of sperm head), Tail Length

—

Contacts

Public ContactHiroki Takeuchi

Mie University

h-takeuchi@med.mie-u.ac.jp+81-592321111

Outcome results