Investigation of Anabolic and Catabolic Responses After a Single Bout of Exercise in Healthy Adults

Investigation of Anabolic and Catabolic Responses After a Single Bout of Exercise in Healthy Adults: Evaluation Using Urinary 3-Methylhistidine - Acute Exercise Muscle Metabolism Study

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

JPRN

Registry ID

JPRN-UMIN000060824

Enrollment

Registered

2026-04-01

Start date

2026-04-01

Completion date

Unknown

Last updated

2026-06-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy adults

Interventions

Exercise Program Contents 1. Aerobic exercise: 40 minutes on a leg ergometer at 65% of peak VO2. 2. Resistance exercise: Bench press, bent-over row, squat, calf raise, 3 sets of 10 repetitions at 70

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: Adults aged 20 or over who are independent in activities of daily living

Exclusion criteria

Exclusion criteria: Those with neurological symptoms, musculoskeletal diseases, or cardiorespiratory diseases that restrict daily life or work, those suspected of cognitive decline, pregnant women, smokers, those taking medication for lifestyle-related diseases such as hypertension, dyslipidemia, or diabetes, those with autoimmune diseases or chronic inflammatory diseases, those taking steroids/NSAIDs/anti-inflammatory supplements on an ongoing basis, those unable to give consent for the study, and others deemed unsuitable by the attending physician.

Design outcomes

Primary

Measure	Time frame
3-Methylhistidine in Urine 24-hour urine collection before and after exercise	—

Secondary

Measure	Time frame
Physical characteristics: age, height, weight, gender, exercise habits, skeletal muscle mass Physical activity (ActiGraph): Sleep assessment (ActiGraph): Dietary intake: BDHQ Blood sampling: Interleukin-6 (IL-6) Interleukin-10 (IL-10) Interleukin-15 (IL-15) Insulin-like growth factor-1 (IGF-1) Tumor necrosis factor (TNF) Brain-derived neurotrophic factor (BDNF) Blood amino acids Transthyretin Retinol-binding protein Albumin Serum creatinine Estimated glomerular filtration rate (eGFR) Blood urea nitrogen Creatine kinase C-reactive protein Cortisol Hemoglobin Hematocrit Urine collection: Urinary creatinine Urinary urea nitrogen Urinary specific gravity Blood sampling: Before exercise, immediately after exercise, 1 hour after exercise, and 24 hours after exercise. Urine collection: 24-hour urine collection performed before and after the exercise session. Physical activity and sleep assessment: Continuous monitoring starting one week prior to the exercise session. Physical characteristics and dietary intake assessment: Assessed prior to the exercise session.	—

Measure

Time frame

Physical characteristics: age, height, weight, gender, exercise habits, skeletal muscle mass Physical activity (ActiGraph): Sleep assessment (ActiGraph): Dietary intake: BDHQ Blood sampling: Interleukin-6 (IL-6) Interleukin-10 (IL-10) Interleukin-15 (IL-15) Insulin-like growth factor-1 (IGF-1) Tumor necrosis factor (TNF) Brain-derived neurotrophic factor (BDNF) Blood amino acids Transthyretin Retinol-binding protein Albumin Serum creatinine Estimated glomerular filtration rate (eGFR) Blood urea nitrogen Creatine kinase C-reactive protein Cortisol Hemoglobin Hematocrit Urine collection: Urinary creatinine Urinary urea nitrogen Urinary specific gravity Blood sampling: Before exercise, immediately after exercise, 1 hour after exercise, and 24 hours after exercise. Urine collection: 24-hour urine collection performed before and after the exercise session. Physical activity and sleep assessment: Continuous monitoring starting one week prior to the exercise session. Physical characteristics and dietary intake assessment: Assessed prior to the exercise session.

—

Countries

Japan

Contacts

Public ContactShinji Kawasaki

Wakayama Medical University Hospital Division of Rehabilitation

shinjik@wakayama-med.ac.jp073-441-0666

Outcome results

None listed

Source: JPRN (via WHO ICTRP) · Data processed: Jul 3, 2026