None
Conditions
Interventions
Brushing teeth with toothpaste for 5 minutes.
Sponsors
Lion corporation
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Subjects who brush their teeth at least twice a day using a toothbrush and toothpaste. Subjects who brush their teeth after dinner and before bedtime. Subjects who have at least 20 teeth remaining. Subjects who collect the necessary amount of saliva for evaluation (>600uL). Subjects who have given voluntary consent in writing to participate in the study.
Exclusion criteria
Exclusion criteria: Subjects who are planning to undergo dental treatment during the study period. Subjects who are planning to participate in oral care-related studies during the study period. Subjects undergoing orthodontic treatment. Subjects who are using bridges, dentures, or implants. Subjects who are diagnosed to have caries (Score 1 or higher). Subjects who are diagnosed with periodontal probing depth of 4mm or more and bleeding on probing. Subjects who have taken antibiotics within the past 3 months. Subjects who smoke. Subjects who regularly drink alcohol more than 5 days a week, exceeding 40g/day for males and 20g/day for females. Subjects who have been diagnosed by a physician as having blood disorders (aplastic anemia, etc.), infectious diseases (varicella, cytomegalovirus infection, measles, infectious mononucleosis), diabetes, renal disease, liver disease, chronic gastritis, colitis, lupus erythematosus, cancer, immune deficiency disease, alcohol dependence, or undernutrition. Subjects who are currently pregnant or have the possibility of pregnancy. Any other subject who, in the opinion of the principal investigator, is not suitable for the study.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Evaluation of the inhibitory effect of saliva on influenza virus cell infection before and after tooth brushing. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Evaluation of saliva before and after tooth brushing. (1) Inhibitory effect of saliva on the interaction between the SARS-CoV-2 spike protein and ACE2. (2) Concentration of IgA binding to SARS-CoV-2 and total IgA. (3) Salivary test data including cariogenic bacteria level, acidity, buffer capacity, leukocyte level, protein level, and ammonia level. 2. Oral examination including Decayed, missing, and filled teeth, probing depths, and bleeding on probing and survey of lifestyle habit. | — |
Countries
Japan
Contacts
Public ContactKei Kurita
Lion corporation Advanced oral health science research laboratories
Outcome results
None listed