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Research to search biomarkers that can objectively evaluate "stress relief" utilization variation data in the concentration of components in saliva collected before and after the acupuncture treatment.

Exploring biomarkers associated with acupuncture-induced changes in human salivary components and stress relief. - Exploring Biomarkers Associated with Acupuncture treatment and Stress Relief

Status
Active, not recruiting
Phases
Unknown
Study type
Interventional
Source
JPRN
Registry ID
JPRN-UMIN000049593
Enrollment
160
Registered
2022-11-23
Start date
2023-01-23
Completion date
Unknown
Last updated
2026-06-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Rehabilitations

Interventions

Group 1: control group (Acupuncture needles, but not electricity) Group 2: 100 HZ-0.2 mA MST (EA) stimulation group Group 3: 100 HZ-1.0 mA EA Stimulation group Group 4: 100 HZ-2.0 mA EA Stimulatio

Sponsors

Faculty of Science and Technology, Kochi University
Lead Sponsor

Eligibility

Sex/Gender
All

Inclusion criteria

Inclusion criteria: The preliminary study (exercise stimulus) at Kochi University is conducted with healthy subjects (20 years and older) who have never smoked. In addition, an exercise protocol (jump rope) is introduced and implemented, which was found to cause fluctuations in blood and saliva components in a preliminary literature review. Prof. Tatsuya Hisajima, Teikyo Heisei University, randomly selects healthy subjects over 20 years old who are undergo electroacupuncture stimulation, saliva collection before and after the stimulation, and a medical questionnaire. On the day of the session, the participants will undergo a health checkup, and then undergo electroacupuncture stimulation at the trigeminal nerve in the frontal lobe, saliva sampling, and a medical questionnaire.

Exclusion criteria

Exclusion criteria: 1.Those who are currently receiving drug treatment for any diseases (except for headache, common cold, etc.). 2.Those who are currently in the habit of taking or applying drugs. 3.Those who have hypersensitive scalp. 4. Those with a history or current history of hypertension, dyslipidemia, or diabetes mellitus. 5. Those with a history or current history of heart failure, renal failure, hepatitis B or hepatitis C 6. Those with a history or current history of serious disorders of the liver, kidneys, heart, lungs, blood, etc. 7. Those who sleep short hours (less than 5 hours a day) or long hours (more than 10 hours a day). 8. Those who have irregular or reversed day and night life due to work or part-time job. 9. Those with a BMI (kg/m2) of 30 or higher. 10. Those who drink more than 60g of alcohol/day on average on a daily basis. 11. Smokers. 12. Those who are likely to change their lifestyle during the study period (e.g., long trips, etc.). 13. Patients who need to take medication during the study period. 14. Currently participating in another human clinical trial, or not yet 3 months have passed since participation in another human clinical trial.

Design outcomes

Primary

MeasureTime frame
This study searchs the indicator substances from salivary components (organic molecules, electrolytes, etc.) that show a strong correlation with cerebral blood flow, which fluctuates before and after acupuncture stimulation.

Secondary

MeasureTime frame
The explored indicator substances will be extensively examined from the viewpoints of neurology, physiology and immunology.

Countries

Japan

Contacts

Public ContactMasanobu Mori

Kochi University Faculty of Science and Technology

mori@kochi-u.ac.jp088-844-8306

Outcome results

None listed

Source: JPRN (via WHO ICTRP) · Data processed: Jul 3, 2026