Impact of inflammatory cytokines to CYP3A or OATP1B activities in rheumatoid arthritis patient using endogenous substrate.

Impact of inflammatory cytokines to CYP3A or OATP1B activities in rheumatoid arthritis patient using endogenous substrate. - Impact of inflammatory cytokines to CYP3A or OATP1B activities in rheumatoid arthritis patient using endogenous substrate.

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

JPRN

Registry ID

JPRN-UMIN000034715

Enrollment

100

Registered

2018-11-12

Start date

2019-01-14

Completion date

Unknown

Last updated

2026-06-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

rheumatoid arthritis

Interventions

None listed

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: RA patients who are not in remission state with DAS 28-CRP (more than 2.3)

Exclusion criteria

Exclusion criteria: Not applicable

Design outcomes

Primary

Measure	Time frame
The prospective study will recruit 100 inpatients or outpatients for 18 years of age or over introduced to the Department of Rheumatology, Oita University Faculty of Medicine, who are not in remission state with DAS 28-CRP score (more than 2.3). Genetic polymorphisms of CYP3A or OATP1B was evaluated by identifying genotypes of single nucleotide polymorphism A6986G (CYP3A53) or A388G (SLOC1B11b) and T521C (SLOC1B15), respectively, using real-time PCR. The concentration of 4beta-OHC in plasma was quantified using gas chromatography-mass spectrometry (GC-MS) previously developed. The plasma CP-I concentration was determined using an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The concentrations of inflammatory cytokines (IL-6, TNF-alpha, IL-1beta) in the plasma are measured using each ELISA. The correlation between inflammatory cytokines and plasma 4beta-OHC or CP-I concentration is statistically analyzed using Pearson correlation coefficient or Spearman rank correlation coefficient. Furthermore, using genotypes of CYP3A53 or SLOC1B11b and SLOC1B15, and background, the major factors that influences these concentrations are analyzed by multivariate logistic regression analysis.	—

Measure

Time frame

The prospective study will recruit 100 inpatients or outpatients for 18 years of age or over introduced to the Department of Rheumatology, Oita University Faculty of Medicine, who are not in remission state with DAS 28-CRP score (more than 2.3). Genetic polymorphisms of CYP3A or OATP1B was evaluated by identifying genotypes of single nucleotide polymorphism A6986G (CYP3A5*3) or A388G (SLOC1B1*1b) and T521C (SLOC1B1*5), respectively, using real-time PCR. The concentration of 4beta-OHC in plasma was quantified using gas chromatography-mass spectrometry (GC-MS) previously developed. The plasma CP-I concentration was determined using an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The concentrations of inflammatory cytokines (IL-6, TNF-alpha, IL-1beta) in the plasma are measured using each ELISA. The correlation between inflammatory cytokines and plasma 4beta-OHC or CP-I concentration is statistically analyzed using Pearson correlation coefficient or Spearman rank correlation coefficient. Furthermore, using genotypes of CYP3A5*3 or SLOC1B1*1b and SLOC1B1*5, and background, the major factors that influences these concentrations are analyzed by multivariate logistic regression analysis.

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Countries

Japan

Contacts

Public ContactRyota Tanaka

Oita University Hospital Department of Clinical Pharmacy

rtanaka@oita-u.ac.jp0975866113

Outcome results