Infertility patients with poor response to gonadotropins
Conditions
Interventions
In situ incision of ovarian cortex 1) Correct serum from the patients before surgery to measure molecular markers to ensure suppression of Hippo signaling. 2) We directly cut ovarian cortex or scrat
Sponsors
Saint Mother Hospital
Eligibility
Sex/Gender
Female
Inclusion criteria
Inclusion criteria: All following four features must be present: 1) Advanced maternal age (=>40 years) or any other risk factor for POR 2) A previous POR (<=3 oocytes with a conventional stimulation protocol) 3) An abnormal ovarian reserve test (i.e. AFC, 5-7 follicles or AMH, 0.5-1.1 ng/ml). 4) Married women
Exclusion criteria
Exclusion criteria: 1)Patients with severe ovarian dysfunction who can not retrieve oocytes after ovarian stimulation. 2)Patients with high risk for laparoscopy. 3)anovulatory amenorrhea patient 4)Male infertility patients with Y chromosome microdeletion and chromosome abnormalities. 5)Patients who can not obtain written informed consent. 6)Patients judged to be inappropriate for the study by the physicians.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Comparison of the number of oocytes retrieved after ovarian stimulation with those of base line numbers. | — |
Secondary
| Measure | Time frame |
|---|---|
| Comparison of proportions of fertilization, pregnancy and abortion with those of base line levels. Comparison of the levels of molecular markers reflecting the results of Hippo signal suppression in ovarian tissues and serum before and after culture and grafting operation, respectively. Also, we count the number of secondary follicles in grafting tissues by histological analysis. To evaluate the effect of in situ incision of ovarian cortex for secondary follicle growth, we measure the levels of serum AMH and inhibin B derived from granulosa cells of secondary follicles. Based on these data, we attempt to identify a suitable time point for initiation of ovarian stimulation. | — |
Countries
Japan
Contacts
Public ContactAtsushi Tanaka
Saint Mother Hospital Medical office
Outcome results
None listed