Genome and epigenome analysis of circulating free DNA and RNA-based liquid biopsy

Genome and epigenome analysis of circulating free DNA and RNA-based liquid biopsy - Genome and epigenome analysis of circulating free DNA and RNA-based liquid biopsy

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

JPRN

Registry ID

JPRN-UMIN000027722

Enrollment

Registered

2017-06-12

Start date

2017-06-14

Completion date

Unknown

Last updated

2026-06-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy adult astronauts

Interventions

None listed

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: Flight duration longer than 3 months

Exclusion criteria

Exclusion criteria: Avoid high-intensity exercise, muscle biopsy and sleep shift

Design outcomes

Primary

Measure	Time frame
After specimen collection and coding, plasma will be isolated from blood. DNA and RNA will be extracted from plasma which contains extra-cellular DNA and RNA circulating in blood stream. For pre-flight samples, additional DNA will be extracted from blood clot which remains in the sampling tube after removal of plasma. This genomic DNA will be used to read reference DNA sequence. By comparing DNA sequence obtained from in-flight plasma DNA sample with reference DNA, presence of DNA damage can be detected during the in-flight and post-flight time course samples.	—

Measure

Time frame

After specimen collection and coding, plasma will be isolated from blood. DNA and RNA will be extracted from plasma which contains extra-cellular DNA and RNA circulating in blood stream. For pre-flight samples, additional DNA will be extracted from blood clot which remains in the sampling tube after removal of plasma. This genomic DNA will be used to read reference DNA sequence. By comparing DNA sequence obtained from in-flight plasma DNA sample with reference DNA, presence of DNA damage can be detected during the in-flight and post-flight time course samples.

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Countries

Japan,North America,Europe

Contacts

Public ContactMasafumi Muratani

University of Tsukuba Faculty of Medicine

muratani@md.tsukuba.ac.jp029-853-7645

Outcome results