Innate and adaptive immune response in subjects with West Nile virus and Usutu virus infections Infections and Infestations
Conditions
Interventions
This is an in vitro study on biological fluids to investigate the adaptive humoral and cell-mediated immune responses in blood donors with West Nile virus (WNV) and Usutu virus (USUV) infections. The
Sponsors
University of Padua
Eligibility
Sex/Gender
All
Age
18 Years to 60 Years
Inclusion criteria
Inclusion criteria: 1. Male or female individuals aged between 18 and 60 years 2. WNV/USUV infection detected through WNV NAT testing as part of blood donor screening 3. Individuals without other acute infections or active chronic diseases, apart from WNV or USUV infection 4. Willingness to provide written informed consent after being adequately informed about the risks and benefits of the study 5. Availability to participate in at least two scheduled study visits
Exclusion criteria
Exclusion criteria: 1. Any individual who, in the investigator's opinion, does not comply with the study objectives and protocol 2. Anticipated absence from the study center during the study period, for example, due to a planned vacation
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Quantification of neutralizing antibody titers by PRNT and MNT at 1, 7, 40 days, and 6 and 12 months post-infection 2. Detection of WNV- and USUV-specific antigen antibodies by EIA at 1, 7, 40 days, and 6 and 12 months post-infection 3. Assessment of cross-reactivity against other orthoflaviviruses by EIA and neutralization assays at 1, 40 days, and 12 months post-infection 4. Analysis of CD4+ and CD8+ T lymphocyte responses measured using flow cytometry at 1 day and 12 months post-infection | — |
Secondary
| Measure | Time frame |
|---|---|
| There are no secondary outcome measures | — |
Countries
Italy
Contacts
Public ContactLuisa Barzon
Outcome results
None listed