Growth of skeletal muscle in response to feeding specific amino acids

Branched-chain amino acid ingestion stimulates muscle myofibrillar protein synthesis following resistance exercise in humans

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN98737111

Enrollment

Registered

2016-12-14

Start date

2009-10-01

Completion date

Unknown

Last updated

2019-11-25

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Resistance exercise Not Applicable

Interventions

This research project was performed in two phases. The design, all procedures, risks and analyses of each phase were the same between the two phases. The only difference was the substances that were i

Eligibility

Sex/Gender

Male

Inclusion criteria

Inclusion criteria: 1. Male, aged 18-45 years 2. Healthy (no known metabolic disorder) 3. Experienced weight lifter for more than 6 months (at least 2 leg resistance training sessions per week)

Exclusion criteria

Exclusion criteria: Not simultaneously taking part in another scientific/clinical study

Design outcomes

Primary

Measure	Time frame
Fractional synthetic rate (FSR), measured using gas combustion isotope ratio chromatography mass spectrometry (GCCIRMS) over the 4h period following drink ingestion.	—

Secondary

Measure	Time frame
1. Signalling proteins, measured using western blots 2. Amino acids in blood, measured using gas chromatography mass spectrometry (GCMS) 3. Tracers enrichment in muscle, measured using GCMS 4. Tracers enrichment in plasma, measured using GCMS 5. Insulin in serum, measured using enzyme-linked immunosorbent assay (ELISA) 6. Glucose in plasma, measured using automated analysers Blood sample timepoints: -240, -145, -85, -25, 0, 15, 30, 45, 60, 75, 90, 120, 150, 180, 240 min Muscle biopsy timepoints: 0, 60 and 240 min	—

Measure

Time frame

1. Signalling proteins, measured using western blots 2. Amino acids in blood, measured using gas chromatography mass spectrometry (GCMS) 3. Tracers enrichment in muscle, measured using GCMS 4. Tracers enrichment in plasma, measured using GCMS 5. Insulin in serum, measured using enzyme-linked immunosorbent assay (ELISA) 6. Glucose in plasma, measured using automated analysers Blood sample timepoints: -240, -145, -85, -25, 0, 15, 30, 45, 60, 75, 90, 120, 150, 180, 240 min Muscle biopsy timepoints: 0, 60 and 240 min

—

Countries

United Kingdom

Contacts

Public ContactSarah Jackman

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026