Obesity, prediabetes Nutritional, Metabolic, Endocrine Obesity
Conditions
Interventions
One hour lasting training sessions, preceded by 10 min warm up and followed by cool down - stretching exercises, are to be held 3 times per week. Sessions of aerobic dance, running and spinning with e
Sponsors
Institute of Experimental Endocrinology Slovak Academy of Sciences (Slovakia)
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Men and women 30-40 years of age 2. Signed written informed consent 3. Volunteers with BMI 30-35 kg/m2 fulfilling the criteria of impaired glucose tolerance (IGT) or impaired fasting glucose (IFG) without pharmacotherapy 4. Lean healthy volunteers (the effect of acute exercise bout)
Exclusion criteria
Exclusion criteria: 1. Acute infectious disease 2. Chronic systemic diseases (cardiovascular disease, chronic diseases of liver and kidneys, diabetes mellitus, oncologic disease or other serious disease, based on the assessment of the responsible physician and investigator) 3. Non-compliance of the volunteer
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Specific molecular changes in spectrum of muscle secreted products (proteins & miRNA) with acute bout of exercise and with the exercise training | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Maximal aerobic capacity (VO2max) will be calculated from the continuous measurement of the gas exchange (Ergostik, Geratherm Respiratory, Bad Kissingen, Germany) during an incremental exercise test (Lode-Corival cycle ergometer, Lode B.V., Groningen, The Netherlands) and expressed relative to lean body mass. 2. Lean body mass 3. Body weight and height will be used to calculate BMI (kg.m-2). 4. Waist circumference will be measured at the midpoint between the lower border of the rib cage and the iliac crest. 5. Bioelectric impedance will be used to evaluate total and visceral adiposity and to estimate lean body mass (Omron BF511, Omron Healthcare LTD., Matsusaka, Japan). 6. Resting energy expenditure will be measured after an overnight fast and following 30min bed rest with the Ergostik (Geratherm Respiratory, Bad Kissingen, Germany) for a period of 30 minutes. 7. Volume and dynamics/intensity of daily ambulatory activity will be determined with accelerometers (Lifecorder plus, Kenz, USA) used continually within the 3-month exercise intervention period. 8. Abdominal fat distribution will be measured in all individuals (both studies) by MRI (1.5 T Magnetom Symphony MRI scanner, Siemens, Germany). 9. Imaging of the calf muscle will enable exact calculation of muscle volume and spectra from tibialis anterior (postural oxidative) muscle acquired by using 1H-MR spectroscopy, used to asses content of intra- and extramyocellular lipids. 10. Hepatic lipid content will be determined by similar 1H MRS technique (as described above). 11. Resting-state muscle ATP turnover with 31P-MRS (magnetization transfer experiment) 12. Level of glucose intolerance using oral glucose tolerance test 13. Fasting plasma glucose, insulin, total and HDL cholesterol, triglycerides and free fatty acids | — |
Countries
Slovakia
Outcome results
None listed