De novo lipogenesis (DNL) and post-prandial TAG levels, which are an established independent risk factor for cardiovascular disease. Nutritional, Metabolic, Endocrine
Conditions
Interventions
This is an acute dietary intervention study with a randomised crossover design, involving two post-prandial metabolic study days.
Participants are randomised following recruitment usin
Sponsors
University of Surrey
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: 1. Gender: Male 2. Ethnicity: Caucasian 3. Age: 18 – 50 years 4. BMI: 25 – 32 kg/m² 5. < 3 sessions of aerobic exercise per week 6. Weight stable for the previous 3 months (± 2.5 kg) 7. Normal blood haemoglobin content (13.5-17.5 g/dL) 8. Blood pressure below 160/100 mmHg (systolic/diastolic)
Exclusion criteria
Exclusion criteria: 1. Fasting plasma glucose > 7 mmol/L 2. Fasting plasma TAG > 4 mmol/L 3. Smoker 4. Type 1 or type 2 diabetes 5. Prescribed lipid-lowering medications 6. Endocrinological, cardiovascular, gastrointestinal and liver diseases or renal impairment 7. History of eating disorders, nausea or vomiting 8. Alcohol intake > 32 g/day or history of drug abuse 9. Relevant food allergies or intolerances e.g. fructose
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| CM-TAG production rate and clearance rate, measured at using intravenous stable isotope labelled glycerol (2H5-glycerol) administered during the post-prandial steady-state during each study visit | — |
Secondary
| Measure | Time frame |
|---|---|
| Measured during by hourly blood sampling at each post-prandial study visit: 1. VLDL-TAG production rate and clearance rate (measure using stable isotope 2H5-glycerol infusion) 2. Rate of intestinal (CM) and hepatic (VLDL) de novo lipogenesis (measured using oral 2H2O) 3. Post-prandial TAG concentration (measured via enzymatic assay) | — |
Countries
United Kingdom
Contacts
Public ContactMargot Umpleby
Outcome results
None listed