Obesity Nutritional, Metabolic, Endocrine Obesity
Conditions
Interventions
The participants will need to visit the Leicester Diabetes Centre for three study visits (pre-, post- and maintenance), and attend the gym within the centre three times a week, for eight weeks.
Pre-i
walking and/or jogging exercise will be encouraged on the treadmill to promote weight bearing exercise and increased gut transit time. All exercise sessions will be specific to the individual’s abilit
Sponsors
University of Leicester
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: 1. Caucasian Males 2. Aged 25-50 years inclusive 3. BMI 30-40kg/m2 inclusive 4. HbA1c less than 6.5% 5. Completion of an eligibility stress test and approval from an in house clinician according to our standard operating procedures 6. Able to speak and understand English
Exclusion criteria
Exclusion criteria: 1. Unable to understand and speak English 2. Unable to provide written informed consent 3. Diagnosed with type 1 or 2 diabetes 4. Diagnosed with crohn’s disease, celiac disease or irritable bowel syndrome 5. Highly active individuals or individuals meeting current physical activity guidelines (selfreport =2.5 hours per week) Participants should not report exercising two or more times per week for 20minutes or longer over the past six months 6. Patients with orthopaedic limitations, motor neurone disease, stage II hypertension (systolic BP>160 and/or diastolic BP>100mmHg), cardiovascular disease (coronary artery disease, cardiomyopathy, heart failure, cor pulmonale, cardiac dysrhythmias, endocarditis, myocarditis, valvular heart disease, cerebrovascular disease, peripheral arterial disease, congenital heart disease and rheumatic heart disease), pulmonary disease (inflammatory lung disease, obstructive lung disease, chronic obstructive pulmonary disease, emphysema, cystic fibrosis, respiratory tract infections, pleural cavity disease, pulmonary vascular disease) renal disease or chair bound 7. Current smokers 8. Are currently on a calorie restricted/weight loss diet 9. Have changed their dietary habits in the past three months 10. Individuals who have used antibiotics or pre/probitoics in the past three months 11. Weight limit (due to operating scales): maximum 200kgs
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Ratio between bacteroidetes and firmicutes is measured using established qPCR assays on faecal samples collected at baseline and 8 weeks. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Relationship between gut microbiota and inflammation is investigated by measuring inflammatory markers such as CRP, TNF-a and IL-6 on non-fasting blood samples collected at baseline and 8 weeks 2. Concentration of short chain fatty acids in faecal samples is measured using established qPCR assays at baseline and 8 weeks 3. Association between the gut microbiota and physical fitness on appetite hormones is determined by measuring GLP-1, PYY and ghrelin on fasting blood samples collected at baseline and 8 weeks | — |
Countries
United Kingdom
Contacts
Public ContactTatiana Plekhanova
Outcome results
None listed