Effect of detraining on polyphenol absorption from orange juice. Nutritional, Metabolic, Endocrine
Conditions
Interventions
All participants were required to attend the laboratory for 8 hours in the trained state and after having not trained for 7 days. On both visits to the laboratory, participants were given an orange ju
Sponsors
University of Glasgow
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Adults over 18 years 2. Distance runners, triathletes or cyclist training regularly for at least 4 years with least 5 hours of endurance training per week 3. Healthy with normal blood pressure 4. Non-smokers 5. Not taking any drug therapies 6. VO2 max >50 ml/kg body mass
Exclusion criteria
Exclusion criteria: 1. History of gastrointestinal disease 2. Vegetarian 3. Having unstable food habits 4. VO2 max < 50 ml/kg body mass
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To determine the effects of training status of endurance athletes on bioavailability of flavanones in endurance trained individuals. 1. Quantification of flavanone metabolites in the blood and urine: Flavanone metabolites in the urine will be analyzed using a HPLC-MS/MS in the selected reaction monitoring mode. Urine samples will also be used for the measurements of DNA oxidation: 8-Oxo-7,8-dihydroxyguanine will be measured by HPLC with electrochemical detection. Will be measured at baseline and following one week of detraining. | — |
Secondary
| Measure | Time frame |
|---|---|
| To find out whether changes in bioavailability of flavanones are related to changes in biomarkers of inflammation, oxidative stress, plasma lipids and insulin sensitivity. 1. Plasma analyses for cardio-metabolic risk factors: The aliquoted EDTA plasma will be stored at 80oC for later analysis of insulin (Ultrasensitive Insulin ELISA, Mercodia AB, Uppsala, Sweden), glucose (Randox, Northern Ireland, UK), TAG, total and HDL-cholesterol (Roche Diagnostics GmbH, 6 Mannheim). Glucose, TAG, total cholesterol, and HDL-cholesterol concentration will be analysed spectrophotometrically (Cobas Mira Plus, ABX Diagnostics, France). High-sensitivity ELISAs (R&D Systems Inc., Oxon, UK) will be used to measure plasma concentration of IL-6 and TNF-?. Will be meassured at baseline and following one week of detraining. | — |
Countries
United Kingdom
Outcome results
None listed