The effect of Personal Shear Rate Therapy and treadmill running exercise on the activation of molecular markers (bradykinin receptors and downstream NO metabolites and telomerase activity) on circulating peripheral leukocytes

A prospective controlled trial to evaluate the effect of Personal Shear Rate Therapy and treadmill exercise on leukocyte molecular marker expression on DNA, RNA and protein level (bradykinin receptors, telomerase activity, NO metabolites) as well as on physiological parameters (relative Pulse Slope Index) to evaluate the efficiency of training

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN88693704

Enrollment

Registered

2014-05-09

Start date

2014-03-01

Completion date

Unknown

Last updated

2015-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Cardiovascular ischemia Circulatory System

Interventions

The study will involve 26 young healthy volunteers, 18-35 years of age, who are willing to be assigned to any of the study intervention schemes, recruited at the Charité - University Medical Center Be

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: 1. Age 18-35 2. Willing to be assigned to any of the study intervention schemes

Exclusion criteria

Exclusion criteria: 1. Smoking 2. Relevant cardiovascular, endocrine or psychological disease 2.1. Hearing defects 2.2. Cardiomyopathy 2.3. Thrombo-embolic events 2.4. Arterial hypertension 3. Metabolic disorders 3.1. BMI >30 3.2. Diabetes mellitus 3.3. Lipometabolic disorders 4. No current treatment (except for oral contraceptives) 5. Pregnancy

Design outcomes

Primary

Measure	Time frame
1. Leukocyte bradykinin receptor expression patterns (B1R/B2R) measured by qRT-PCR 2. Leukocyte telomerase activity measured by telomeric repeat amplification protocol (TRAP) 3. Plasma NO levels measured by an assay using gries-reagent 4. Relative Pulse Slope Index (rPSI) measured by ultrasound 5. Electrical cardiometry ('Window to the heart' Osypka Medical GmbH) Each outcome will be measured directly before and after exercise and PSRT. Likewise, for molecular analysis, volunteer blood samples are collected before and after exercise and PSRT.	—

Measure

Time frame

1. Leukocyte bradykinin receptor expression patterns (B1R/B2R) measured by qRT-PCR 2. Leukocyte telomerase activity measured by telomeric repeat amplification protocol (TRAP) 3. Plasma NO levels measured by an assay using gries-reagent 4. Relative Pulse Slope Index (rPSI) measured by ultrasound 5. Electrical cardiometry ('Window to the heart' Osypka Medical GmbH) Each outcome will be measured directly before and after exercise and PSRT. Likewise, for molecular analysis, volunteer blood samples are collected before and after exercise and PSRT.

—

Secondary

Measure	Time frame
1. Kallikrein-Kinin system molecule quantification ? Kininogen, Kallikrein, ACE 2. Molecular parameters such as protein expression analysis, DNA analysis, RNA expression quantification of leukocyte subpopulation markers (M1/M2 macrophage marker, Toll-like receptor TLR2/TLR4, shelterin components [TRF1, TRF2], TERT, G-CSF/GM-CSF receptor, endothelial progenitor cell marker CD133, CD34, and VEGFR-2 as well as CCR2 and ligand MCP-1), NO plasma levels, and plasma secretome analysis for endothelial monoculture, as well as endothelial cell and smooth muscle cell co-culture assays before and after PSRT vs control. Measured by qRT-PCR, western blot or ELISA, respectively Each outcome will be measured directly before and after exercise and PSRT. Likewise, for molecular analysis, volunteer blood samples are collected before and after exercise and PSRT.	—

Measure

Time frame

1. Kallikrein-Kinin system molecule quantification ? Kininogen, Kallikrein, ACE 2. Molecular parameters such as protein expression analysis, DNA analysis, RNA expression quantification of leukocyte subpopulation markers (M1/M2 macrophage marker, Toll-like receptor TLR2/TLR4, shelterin components [TRF1, TRF2], TERT, G-CSF/GM-CSF receptor, endothelial progenitor cell marker CD133, CD34, and VEGFR-2 as well as CCR2 and ligand MCP-1), NO plasma levels, and plasma secretome analysis for endothelial monoculture, as well as endothelial cell and smooth muscle cell co-culture assays before and after PSRT vs control. Measured by qRT-PCR, western blot or ELISA, respectively Each outcome will be measured directly before and after exercise and PSRT. Likewise, for molecular analysis, volunteer blood samples are collected before and after exercise and PSRT.

—

Countries

Germany

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026