Metabolic flexibility and maximal aerobic capacity in healthy subjects Nutritional, Metabolic, Endocrine
Conditions
Interventions
In the selected individuals, the maximum aerobic capacity (by indirect calorimetry) and lactate threshold (by concentration in lactate venous blood) in an exercise bicycle will be determined. On a sec
Sponsors
Fondecyt
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: 1. Healthy men (by physical examination, past and current medical history, and routine blood testing, including biochemical profile, thyroid-stimulating hormone, free thyroxine, electrolytes, creatinine, and hemogram) 2. Between 18 and 35 years old 3. IMC: 18.5 – 25 kg/m2 4. Stable body weight (change<2 kg over the past 3 months) 5. Physical activity (<3 days/week) 6. Do not take medications
Exclusion criteria
Exclusion criteria: 1. Men with any disease 2. Younger than 18 years old and older than 35 years old 3. Unstable weight (change>2 kg over the past 3 months) 4. Physical activity (>3 days/wk) 5. Taking medications
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Resting metabolic rate and respiratory quotient, measured by indirect calorimetry at two of three sessions with an interval of 4-7 days during the month following the selection 2. Glucose metabolic flexibility, measured by euglycemic-hyperinsulinemic clamp 3. Maximal aerobic capacity, measured by indirect calorimetry and a blood sample for lactate concentration in venous blood during exercise for 15 minutes. This measurement is performed during the month following the selection | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Glucose, lactate, free fatty acids, triglycerides, VLDL, 3-OH-butyrate and insulin concentration, measured by taking two blood samples with a 10-minute interval before and after the clamp 2. Body mass and body composition, measured using the body mass index formula (BMI) and electrical bioimpedance | — |
Countries
Chile
Outcome results
None listed