Healthy adult volunteers Oral Health
Conditions
Interventions
Salivary secretion capacity will be assessed in all participants at baseline.
The participants will randomly be allocated to the control and intervention arms in equal numbers (22 in each arm):
1. I
Portugal),
Salivary secretion rate and fluoride concentration will be recorded at defined time intervals (before toothbrushing at 0 and 10 minutes
after toothbrushing at 0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 110 and 120 minutes) to determine if the amount of fluoride would be able to induce toxic effects in the human bod
Sponsors
University of Lisbon (Portugal)
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Above 18 years 2. An unstimulated whole saliva flow higher than 0.1 mL/min 3. Healthy volunteers, without any systemic pathologies
Exclusion criteria
Exclusion criteria: 1. Pregnant 2. Medication that induce xerostomia 3. Does not meet inclusion criteria
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Amount of fluoride present in saliva during and after toothpaste use, expressed in milligrams, as the mean ± 95% confidence interval. Fluoride present in the toothbrush, mouthwash and in saliva after toothpaste use, expressed in parts per million will be determined using metric techniques and a fluoride selective electrode. This value will be converted into milligrams, according to each dentifrice and its fluoride concentration. Percentage values will be determined, considering the toothpaste placed on the toothbrush (expressed in grams, and calculated from the difference of weight of the toothbrush before and after toothpaste placement) as the total amount of fluoride, meaning 100% of fluoride of exposure. All the fluoride accumulated in saliva will be determined and, based on this value, calculation will be performed for each dentifrice to determine how many times a patient would have to brush his teeth per day to cause any deleterious effect, according to the reference values for acute and chronic fluoride toxicity in the literature. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Concentration of fluoride present in saliva before, during and after toothpaste use, expressed in parts per million, as the mean ± 95% confidence interval 2. Amount of paste placed in the toothbrush, expressed in grams, as the mean ± 95% confidence interval 3. Fluoride wasted in the toothbrush and in the mouthwash, expressed in grams, as the mean ± 95% confidence interval 4. Fluoride wasted in the toothbrush and in the mouthwash, expressed in ppm, as the mean ± 95% confidence interval 5. Fluoride present in the toothbrush, mouthwash and in saliva after toothpaste use, expressed in percentage, as the mean ± 95% confidence interval 6. Salivary flow after brushing with the dentifrices, expressed in milliliters per minute, as the mean ± 95% confidence interval | — |
Countries
Portugal
Outcome results
None listed