Efficacy of 0.1% chlorine dioxide mouthwash in reducing oral malodour

Efficacy of 0.1% chlorine dioxide mouthwash in reducing oral malodour – a 2-week randomized double-blind cross-over study

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN75902618

Enrollment

Registered

2018-03-15

Start date

2017-02-01

Completion date

Unknown

Last updated

2020-08-10

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Oral malodour/halitosis Oral Health Halitosis

Interventions

The experimental sample was commercial mouthwash (TheraBreath® Mild Mint Oral Rinse) containing 0.1% chlorine dioxide. The control sample was 0.9% sodium chloride solution with additional flavours to

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: 1. First- to third-year students at the University of Medicine and Pharmacy, Ho Chi Minh City who had halitosis as a chief complaint 2. An organoleptic score = 2 based on the Rosenberg scale (Rosenberg & McCulloch, 1992) 3. A level of H2S > 1.5 ng/10 mL or CH3SH > 0.5 ng/10 mL (1) determined by OralChromaTM

Exclusion criteria

Exclusion criteria: 1. Gastrointestinal diseases or respiratory diseases 2. Habit of smoking 3. Wearing dentures or orthodontic appliances 4. Undergoing any antibiotic treatment 1 month before and during the study course

Design outcomes

Primary

Measure	Time frame
VSC concentration measured with hydrogen sulphide (H2S) and methyl mercaptan (CH3SH) gas analysis machine at baseline, 12 hours and 2 weeks	—

Secondary

Measure	Time frame
1. Organoleptic score measured directly by an examiner using 0-5 scale at baseline, 12 hours and 2 weeks 2. Plaque index (PI) and gingival index (GI) assessed using the method of Loe and Silness (Loe, 1967), and bleeding on probing (BOP) evaluated at four sites (distal, buccal, mesial and lingual) on all teeth except for third molars at baseline, 12 hours and 2 weeks 3. Evaluation of tongue coating based on the criteria of Winkel et al. (2003) at baseline, 12 hours and 2 weeks The pH of resting saliva determined by a pH paper test (Saliva-Check Buffer Kit, GC, Japan) at baseline, 12 hours and 2 weeks 4. Detection and determination of bacterial species A. actinomycetemcomitans, F. nucleatum, P. gingivalis, S. moorei, S. salivarius, T. denticola and T. forsythia in resting saliva using a multiplex real-time polymerase chain reaction (PCR) assay at baseline and after 2 weeks	—

Measure

Time frame

1. Organoleptic score measured directly by an examiner using 0-5 scale at baseline, 12 hours and 2 weeks 2. Plaque index (PI) and gingival index (GI) assessed using the method of Loe and Silness (Loe, 1967), and bleeding on probing (BOP) evaluated at four sites (distal, buccal, mesial and lingual) on all teeth except for third molars at baseline, 12 hours and 2 weeks 3. Evaluation of tongue coating based on the criteria of Winkel et al. (2003) at baseline, 12 hours and 2 weeks The pH of resting saliva determined by a pH paper test (Saliva-Check Buffer Kit, GC, Japan) at baseline, 12 hours and 2 weeks 4. Detection and determination of bacterial species A. actinomycetemcomitans, F. nucleatum, P. gingivalis, S. moorei, S. salivarius, T. denticola and T. forsythia in resting saliva using a multiplex real-time polymerase chain reaction (PCR) assay at baseline and after 2 weeks

—

Countries

Viet Nam

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026