Tendon protein synthesis in otherwise healthy males and females scheduled for anterior cruciate ligament (ACL) surgery. Injury, Occupational Diseases, Poisoning
Conditions
Interventions
The study involves two parallel groups: an intervention group and a control group:
Intervention: A vitamin C-containing collagen supplement (20 g per dose) will be ingested as a drink (100 mL of water
Sponsors
HAN University of Applied Sciences
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Otherwise healthy male and female volunteers 2. Aged 18 to 40 years old 3. Recruited from patients attending the Department of Orthopedics from the participating hospital 4. Planned for ACL reconstruction with hamstring autograft 5. BMI =18.5 and =27.5 kg/m2
Exclusion criteria
Exclusion criteria: 1. Blood donation during the study period or during the last 2 months 2. Pregnancy 3. Third-generation oral contraceptives 4. Currently smoking 5. Consumption of >21 alcoholic beverages per week 6. Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications) 7. Regular use of protein or other nutritional supplements (including vitamins). 8. Reported slimming or medically prescribed diet 9. Use of antibiotics in the past month 10. Current participation in another biomedical research study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Tendon tissue protein synthesis rate, expressed as fractional synthesis rate (FSR, %/d), will be measured for 7 days prior to surgery. To calculate FSR, the standard precursor-product method will be used. Tendon protein synthesis rates will be calculated from: 1. Saliva 2H enrichment (i.e., precursor) 2. Tissue protein-bound enrichment 2H-alanine 3. Plasma albumin enrichment 2H-alanine | — |
Secondary
| Measure | Time frame |
|---|---|
| A ligament in muscle protein synthesis rate, expressed as fractional synthesis rate (FSR, %/d), will be measured for 7 days prior to surgery. To calculate FSR, the standard precursor-product method will be used. Tendon protein synthesis rates will be calculated from: 1. Saliva 2H enrichment (i.e., precursor) 2. Tissue protein-bound enrichment 2H-alanine 3. Plasma albumin enrichment 2H-alanine | — |
Countries
Netherlands
Contacts
Public ContactJan-Willem van Dijk
Outcome results
None listed