Antioxidant supplementation, oxidative stress, Heat Shock Protein (HSPs), antioxidant enzyme Not Applicable
Conditions
Interventions
Main Experimental Trials: Participants will be randomly assigned to either consume a tablet of 1000 mg Quercetin (Q), Quercetin plus vitamin C (QC) or placebo control (CON) 14 hours (the night before
Sponsors
University of Bath (UK)
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: 1. Males 2. Aged between 18-48 years 3. Individuals free from cardiovascular, metabolic or joint disease as determined by a standard health questionnaire 4. Non-smoker 5. Not taking any mineral or vitamin supplement (other than those provided) or any other antioxidant supplements for 2 weeks before and during the study
Exclusion criteria
Exclusion criteria: 1. Individuals taking vitamin supplements 2. Any bleeding disorder or taking medication which impacts blood coagulation 3. Known tendency towards keloid scarring 4. Known sensitivity or allergy to any local anaesthetic medicines 5. Any reported use of substance which may pose undue personal risk to participants or introduce bias into the experiment 6. Any other condition or behaviour deemed either to pose undue risk to participants or introduce bias into the experiment 7. A known renal dysfunction
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Oxidative stress blood markers and intramuscular heat shock protein (HSPs) following each experimental trial. Blood antioxidant measurements: quercetin, ascorbic acid, urate, oxidised/reduced glutathione (GSSG-GSH) ratio, total antioxidant capacity and oxidative stress biomarkers (lipid peroxidation (MDA) and protein carbonyl). Measured before warm-up, immediately after warm-up, every 20 minutes during the one hour exercise bout and post one hour exercise. | — |
Secondary
| Measure | Time frame |
|---|---|
| Intramuscular heat shock proteins (HSP 70 and HSP60), measured 2 days pre-exercise and 2 days post-exercise | — |
Countries
United Kingdom
Outcome results
None listed