The role of lung inflation on neutrophil function in patients undergoing coronary artery bypass grafting Circulatory System
Conditions
Interventions
Eligible patients will be adults having coronary artery bypass grafting (CABG) at the Freeman Hospital, Newcastle upon Tyne. A full explanation of what is involved will be given when the patients are
Sponsors
Newcastle Upon Tyne Hospitals NHS Foundation Trust (UK)
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Aged 18 or over 2. Patients undergoing coronary artery bypass graft surgery
Exclusion criteria
Exclusion criteria: 1. Patients under 18 years of age 2. Lack of informed, written consent 3. Emergency surgery precluding the capacity to give sufficient time for informed written consent 4. Redo surgery 5. Patients taking part in other interventional studies of CTIMPs that may significantly affect white blood cell function
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Differences in phagocytic capacity of neutrophils in blood is measured by way of direct microscopic visualisation of ingestion of Zymosan particles and by flow cytometric analysis using staph. aureus PHrodo bioparticles at baseline, during and after CPB. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Differences in neutrophil priming status is measured by differences in cell surface expression markers (CD11b and CD62L) and release of reactive oxygen species by flow cytometric analysis at baseline, during and after CPB 2. Differences in the serum cytokines of blood is measured using an ELISA assay at baseline, during and after CPB | — |
Countries
England, United Kingdom
Contacts
Public ContactWendy Funston
Outcome results
None listed