Oxidative stress in healthy subjects Not Applicable
Conditions
Interventions
1. One single dose (one glass) of red wine, dealcoholised red wine or water in a fasting state (Single dose analysis) OR
2. One glass of red wine, dealcoholised red wine or no special drink (controls)
Sponsors
Institute for Molecular Biotechnology (Germany)
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Healthy, normal-weight male and female subjects between 18 and 50 years of age.
Exclusion criteria
Exclusion criteria: 1. Pregnancy and lactation: women not taking oral contraceptives underwent a pregnancy test to exclude an unknown pregnancy before starting the intervention 2. Known diseases of the liver and pancreas or dysfunctions of the gastro-intestinal tract associated with maldigestion or malabsorption 3. Hypertension 4. Dysfunctions of the lipid metabolism 5. Hyperuricaemia/gout 6. Kidney dysfunctions 7. Autoimmune diseases 8. Diabetes mellitus type I or type II 9. Acute infectious diseases 10. Eating disorders 11. Allergy to hen's eggs (albumen used as finings in red wine) 12. Alcohol abuse (more than 40 g/day) or previous addiction to alcohol 13. Drug abuse 14. Tobacco smoking during the last 6 months prior to the study 15. Regular intake of pharmaceuticals, which influence the immune system and/or the antioxidant capacity in plasma (anti-inflammatory drugs including cortisol containing medications, immune stimulants) as well as intake of drugs which might cause adverse affects by interacts with alcohol 16. Intake of nutritional supplements e.g. multivitamins, supplements containing vitamin A, C and/or vitamin E, fish oil or red wine preparations, bioactive concentrates 17. Excessive exercising (competitive athletes) defined as greater than 10 hours strenous exercise per week 18. Limited contractual capability 19. Every other state opposed to participation in the study stated by the medical supervisor 20. Participation in any other clinical trial (ongoing or completed less than 30 days prior to the present study)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Concentration of total phenolic compounds in plasma (folin assay) 2. Deoxyribonucleic acid (DNA) strand breaks in peripheral leukocytes (single cell gel electrophoresis) 3. Antioxidant capacity in plasma (trolox equivalent antioxidant capacity) 4. Apoptosis of PHA activated T lymphocytes (annexin-V binding test) 5. Phagocytosis in monocytes and granulocytes (Phagotest® test kit) 6. Respiratory burst in monocytes and granulocytes (Bursttest® test kit) | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Vitamin C concentration in plasma a-tocopherol concentration in serum (dietary intervention trial only) 2. Concentration of uric acid, albumin and bilirubin in plasma | — |
Countries
Germany
Outcome results
None listed