Effect of probiotic yoghurt on blood indices and intestinal microflora of healthy volunteers

Effect of probiotic yoghurt on blood indices and intestinal microflora of healthy volunteers: a randomised double-blind cross-over trial

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN68198472

Enrollment

Registered

2009-03-06

Start date

2009-03-09

Completion date

Unknown

Last updated

2015-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Intestinal microflora, blood pressure and immunological parameters of healthy adults Not Applicable

Interventions

Probiotic yoghurt consumption versus regular yoghurt consumption. Volunteers are randomly allocated to receive either: 1. 150 g probiotic yoghurt (group 1) or control yoghurt (group 2) once a day for

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: 1. Wish to participate in the study 2. Both males and females, aged 18-65 years 3. Healthy (i.e. no known health problems and no medical conditions that require drug therapy) 4. Signed informed consent

Exclusion criteria

Exclusion criteria: 1. History of any gastrointestinal disease 2. Use of any antimicrobial agent within the preceding month 3. Use of any regular concomitant medication including non-steroidal anti-inflammatory drugs and antioxidant vitamins 4. Pregnancy or breastfeeding 5. Food allergy 6. Diabetes and acute infections

Design outcomes

Primary

Measure	Time frame
The following will be measured at recruitment, after 3-week intervention, after 2-week washout and at the end of another 3-week intervention: 1. Clinical blood indices 2. Intestinal microflora	—

Secondary

Measure	Time frame
The following will be measured at recruitment, after 3-week intervention, after 2-week washout and at the end of the trial i.e. at the end of another 3-week intervention: 1. The self-reported questionnaire containing questions on welfare, nutritional habits, and habitual gastrointestinal symptoms (abdominal pain, flatulence, bloating, and stool frequency) 2. Haematological indices, plasma glucose, total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) cholesterol, triglyceride and high sensitivity C-reactive protein (hsCRP), Interleukin-6 (IL-6), immunoglobulin levels 3. The content of biogenic amines and polyamines in urine 4. Reverse transcription polymerase chain reaction (RT-PCR) from fecal samples for monitoring the changes in the counts of lactoflora, L. planatrum and survival of the administered strains 5. Denaturing gradient gel electrophoresis (DGGE) used to monitor changes in total fecal microflora after yoghurt consumption	—

Measure

Time frame

The following will be measured at recruitment, after 3-week intervention, after 2-week washout and at the end of the trial i.e. at the end of another 3-week intervention: 1. The self-reported questionnaire containing questions on welfare, nutritional habits, and habitual gastrointestinal symptoms (abdominal pain, flatulence, bloating, and stool frequency) 2. Haematological indices, plasma glucose, total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) cholesterol, triglyceride and high sensitivity C-reactive protein (hsCRP), Interleukin-6 (IL-6), immunoglobulin levels 3. The content of biogenic amines and polyamines in urine 4. Reverse transcription polymerase chain reaction (RT-PCR) from fecal samples for monitoring the changes in the counts of lactoflora, L. planatrum and survival of the administered strains 5. Denaturing gradient gel electrophoresis (DGGE) used to monitor changes in total fecal microflora after yoghurt consumption

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Countries

Estonia

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026