Intracytoplasmic sperm injection cases, oocyte vitrification Pregnancy and Childbirth Fertilisation difficulties
Conditions
Interventions
After oocyte denudation, MII oocytes with normal morphology were randomly allocated to fresh ICSI insemination or to vitrification procedure. If pregnancy was not obtained a subsequent ICSI cycle was
Sponsors
G.EN.E.R.A. - Clinica Valle Giulia (Italy)
Eligibility
Sex/Gender
Female
Inclusion criteria
Inclusion criteria: Between September 2008 and February 2009 consecutive patients not older than 42 years of age, presenting greater than 6 normal appearing metaphase II (MII) oocytes and undergoing ICSI treatment with ejaculated sperm in the Centre for Reproductive Medicine GENERA in Rome.
Exclusion criteria
Exclusion criteria: 1. Female partner older than 42 years old 2. Less than 6 normal appearing MII oocytes retrieved 3. Surgically extracted spermatozoa 4. Very severe oligoastenoteratozoospermia (motile sperm count less than 500,000/ml after preparation) 5. Patients enrolled in our polar body biopsy programme
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Non-inferiority in fertilisation rates calculated per warmed and per injected oocyte, assessed 16 - 18 hours post-treatment (ICSI). | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Pronuclear morphology 2. Embryo development, assessed 42 - 44 hours post-treatment 3. Patient's baseline characteristics 4. Clinical outcomes | — |
Countries
Italy
Outcome results
None listed