Myocardial infarction, endothelial disease Circulatory System
Conditions
Interventions
On the day of participation, a physical examination (cardiopulmonary system) will be performed by a physician prior to the start of the investigational intervention.
The investigation
Sponsors
Academic Medical Center (Netherlands)
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: Healthy male volunteers aged 18-45 years
Exclusion criteria
Exclusion criteria: 1. Any cardiovascular, kidney, pulmonary or endocrine diseases 2. Alcohol or drug abuse 3. No informed consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Lactate dehydrogenase enzyme (LDH) release in human umbilical vein endothelial cells measured at different time points in our in vitro model after incubation with the serum. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. We measured mitogen activated protein kinase ERK 1/2, protein kinase B (AKT) and hypoxia inducible factor (HIF) 1 alpha in the endothelial cells by the use of western blotting at time point T0 before remote preconditioning and T1 (after 45 minutes, directly after the remote conditioning protocol). 2. We measured human vascular endothelial growth factor in the plasma of the volunteers also at T0 and T1. | — |
Countries
Netherlands
Outcome results
None listed