Oocyte donation Pregnancy and Childbirth
Conditions
Interventions
The cryopreservation technique used is vitrification and more specifically the closed vitrification system, in order to achieve aseptically conditions. The human oocytes are exposed in solutions with
Sponsors
Iakentro Advanced Medical Center
Eligibility
Sex/Gender
Female
Inclusion criteria
Inclusion criteria: Oocyte donor inclusion criteria: 1. Up to 32 years old 2. Body mass index of less than 30 kg/m2 3. Regular menstrual cycles of 25–35 days 4. Two normal ovaries based on transvaginal scan findings 5. No polycystic ovary syndrome 6. No known endometriosis 7. No gynecological or medical disorders 8. Agreed to donate their oocytes for treatment anonymously and altruistically 9. Known fertility and good ovarian response 10. Blood sample was collected for karyotype and screening for previous viral infection (hepatitis B and C, human immunodeficiency virus, syphilis) thalassemia and cystic fibrosis 11. A single stimulation cycle was included for each donor Recipient inclusion criteria: 1. Up to 50 years old 2. No history of endometriosis 3. First oocyte donation cycle 4. The recipients and their partners underwent blood screening similar to the donors, hysterosalpingogram and a diagnostic hysteroscopy had eliminated cases presenting hydrosalpinx or intrauterine pathology 5. The recipients had a mock transfer in a cycle previous to their donation cycle and if difficulty was encountered a cervical dilatation was performed 6. The recipient’s partner had no severe male infertility indication
Exclusion criteria
Exclusion criteria: Donor exclusion citeria: Less than 15 retrieved mature oocytes Recipient exclusion criteria: 1. More than 2 previous failed oocyte donation cycles 2. Severe male factor infertility in partner
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Pregnancy rate per cycle, confirmed by the rise of serum b-HCG concentrations, 14 days after embryo transfer 2. Clinical pregnancy rate per cycle, defined by the appearance of a gestational sac and a fetal heartbeat at 8–10 weeks of gestation 3. Ongoing pregnancy rate per cycle, considered the number of pregnancies with fetuses displaying heart activity beyond 12 weeks of gestation per cycle | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Oocyte survival rate, defined as the number of oocytes that survived out of the total number of oocytes warmed, is evaluated 2 hours after the warming of the oocytes 2. Fertilization rate, defined as the number of fertilized oocytes out of the number of oocytes survived, evaluated 16-20 hours after the injection of a spermatozoon to the oocytes 3. Cleavage and top cleavage rate, was defined as the number of cleavage embryos, top quality cleavage embryos respectively out of the total number of fertilized oocytes, evaluated 3 days after the injection of a spermatozoon to the oocytes 4. Blastocyst and the top blastocyst rate, defined as the number of blastocysts and top quality blastocysts respectively out of the total number of fertilized oocytes, evaluated 5 days after the injection of a spermatozoon to the oocytes | — |
Countries
Greece
Outcome results
None listed