Diet and the microbiome Nutritional, Metabolic, Endocrine Diet and the microbiome
Conditions
Interventions
This study aims to identify the microbial responders to specific micronutrients in the human diet. By placing a cohort of approximately 50 healthy human individuals on a diet consisting entirely of a
microbiome composition is measured throughout the course of the intervention.
The study involves comparing the effects of a controlled diet in contrast to a variable diet on the compo
Sponsors
MIT Center for Microbiome Informatics and Therapeutics
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Individuals between 18-70 years of age 2. BMI between 18-25 or above 30 3. A waist-to-hip ratio of more than 0.9
Exclusion criteria
Exclusion criteria: 1. Food allergies 2. Inability to comply with study procedure 3. Suffer from IBS, IBD, Type-2 diabetes, kidney disease or intestinal obstruction 4. Allergic to polyethylene glycol 5. Have received antibiotics in the last 6 months 6. Have untreated, in situ colorectal cancer 7. Are currently pregnant, planning to get pregnant in the next 60 days, or currently breastfeeding
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Gut microbiota composition is measured using the stool samples collected on the two first baseline days and on each day during the provided dietary regimen. This consists of a maximum of 10 stool samples (two baseline, six intervention, and two follow-up), which are sequenced for the 16S rRNA gene. | — |
Secondary
| Measure | Time frame |
|---|---|
| The effect of the diet on the microbiota at the level of genes and bacterial strains are processed for metagenomics sequencing at the second baseline sample, day 3 sample, and day 6 sample. | — |
Countries
United States of America
Contacts
Public ContactEric Alm
Outcome results
None listed