Intracytoplasmic sperm injection (ICSI), aseptic open oocyte vitrification in UV-sterilised liquid nitrogen, hermetical cryostorage Pregnancy and Childbirth
Conditions
Interventions
After retrieval oocytes were denuded and then evaluated to assess their nuclear maturation stage. The oocytes that had released the first polar body (metaphase II - MII) underwent a strict selection b
the supernumerary sibling MII oocytes were vitrified.Since at the beginning of this study the Italian IVF law allowed the injection of maximum three oocytes (Benagiano and Gianaroli, 2004), between Ap
from 9 April 2009 the number of warmed oocytes to inject has defined following our centre?s guideline.
LN2 sterilisation via UV irradiation was performed by administration of 660,000 µW/cm2.
Cryoto
Sponsors
GynePro Medical Centers (Italy)
Eligibility
Sex/Gender
Female
Inclusion criteria
Inclusion criteria: 1. Women enrolled in the vitrification programme at GynePro medical centre undergoing ICSI with ejaculated spermatozoa 2. Not older than 41 years 3. At least six mature-Methaphase II oocytes at retrieval This study compares the outcome of 31 warmed ICSI cycles performed from January 2009 to May 2010 with the outcome of fresh sibling oocytes ICSI performed from April 2008 to March 2010
Exclusion criteria
Exclusion criteria: 1. Women older than 41 years old 2. Women with less than six mature-MII oocytes at retrieval 3. Patients with male partners with testicular spermatozoa or severe oligoastenoteratozoospermia (motile sperm count = 500.000/mL after sperm preparation)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Oocyte fertilisation rate 2. Embryo cleavage rate 3. Top-quality embryo rate | — |
Secondary
| Measure | Time frame |
|---|---|
| No secondary outcome measures | — |
Countries
Italy
Outcome results
None listed