Oral hydroxycitric acid (HCA) supplementation enhances glycogen synthesis in exercised human skeletal 1 muscle

Oral hydroxycitric acid (HCA) supplementation enhances glycogen synthesis in exercised human skeletal 1 muscle: a randomised placebo-controlled cross-over trial

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN45782014

Enrollment

Registered

2011-01-21

Start date

2006-08-01

Completion date

Unknown

Last updated

2015-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Glycogen synthesis in human skeletal muscle Nutritional, Metabolic, Endocrine Glycogen synthesis

Interventions

Since we aimed to determine the HCA effect on recovery, HCA and placebo meals were provided to the subjects immediately after exercise. HCA trial provided a meal containing 70% carbohydrate content wi

Eligibility

Sex/Gender

Male

Inclusion criteria

Inclusion criteria: 1. Healthy male volunteers 2. Aged 22.0 +/- 0.3 years 3. Height 171.3 +/- 0.7 cm 4. Weight 73.7 +/- 1.1 kg 5. Body mass index (BMI) 25.2 +/- 0.5 kg/m2 6. Ventilatory equivalent for oxygen (VEO2) 108 7. Peak 45.7 +/- 1.5 ml/kg/min

Exclusion criteria

Exclusion criteria: Male volunteer's VO2max less than 30 ml/kg/min

Design outcomes

Primary

Measure	Time frame
Glycogen assay. Approximately 25 mg of skeletal muscle from the deep portion of the vastus lateralis was dissolved in 1 N KOH at 75°C for 30 minutes. Dissolved homogenate was neutralised by glacial acetic acid and incubated overnight in acetate buffer (0.3 M sodium acetate, pH to 4.8) containing amyloglucosidase (Boehringer Mannheim, Indianapolis, IN). The reaction mixture was neutralised with 1 N NaOH. Samples were then analysed by measuring glucosyl units by the Trinder reaction (Sigma, St. Louis, MO).	—

Measure

Time frame

Glycogen assay. Approximately 25 mg of skeletal muscle from the deep portion of the vastus lateralis was dissolved in 1 N KOH at 75°C for 30 minutes. Dissolved homogenate was neutralised by glacial acetic acid and incubated overnight in acetate buffer (0.3 M sodium acetate, pH to 4.8) containing amyloglucosidase (Boehringer Mannheim, Indianapolis, IN). The reaction mixture was neutralised with 1 N NaOH. Samples were then analysed by measuring glucosyl units by the Trinder reaction (Sigma, St. Louis, MO).

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Secondary

Measure	Time frame
Molecular expressions of GLUT4, FAT/CD36, measured with reverse transcription-polymerase chain reaction (RT-PCR) and western blotting	—

Countries

Taiwan

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026