Evaluation of blastocyst survival following vitrification and thawing using two methods - Fast Freeze™ Kit based on S3 and the Vit. Kit®

Evaluation of blastocyst survival following vitrification and thawing using two methods - Fast Freeze™ Kit based on S3 and the Vit. Kit®: a randomised controlled study

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN41121428

Enrollment

100

Registered

2012-03-01

Start date

2012-03-01

Completion date

Unknown

Last updated

2019-02-11

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Reproductive medicine Urological and Genital Diseases

Interventions

Group 1 Global® Fast Freeze and Thawing media ? based on S31 blastocyst at the time is removed from the incubator and transferred with a micropipette to previously made drops of vitri

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: 1. Blastocysts derived from 1PN and 3PN embryos (abnormal embryos) 2. Blastocysts derived from biopsied and rejected embryo 3. Blastocysts derived from too fast or too slow embryos (that were neither transferred nor cryopreserved) 4. Blastocysts derived from culture of embryos (days 2 and 3) donated for scientific research

Exclusion criteria

Exclusion criteria: Still useful embryos and normal embryos not donated for scientific research

Design outcomes

Primary

Measure	Time frame
1. Survival assessment by vital staining 2. Assessment of cell number and survival rate following vitrification 3. Thawing and culture overnight is performed with the Life-dye TM and propidium iodide (Life-dead cell staining kit; Biovision, CA, US). Briefly blastocysts are incubated in a buffer media with 1ul of each staining solutions for 15 min in the dark, at 37ºC.	—

Secondary

Measure	Time frame
1. Differences in terms of morphology, kinetics and cell survival on the three groups. Digital images of each blastocyst (day 5 or day 6) are acquired before the vitrification procedure, immediately after thawing and after overnight culture, using a digital still camera (Octax, Microscience Gmb H, Bruckberg, Germany) mounted on an inverted optical microscope (TE2000-S, Nikon Eclipse, Japan), with a thermal control microscope stage (MS100, Linkam, Surray, UK). 2. Assessment of blastocyst morphology quality is done according to the classification developed by Gardner & Schoolcraft 1999 and re-expansion is assessed following overnight culture.	—

Measure

Time frame

1. Differences in terms of morphology, kinetics and cell survival on the three groups. Digital images of each blastocyst (day 5 or day 6) are acquired before the vitrification procedure, immediately after thawing and after overnight culture, using a digital still camera (Octax, Microscience Gmb H, Bruckberg, Germany) mounted on an inverted optical microscope (TE2000-S, Nikon Eclipse, Japan), with a thermal control microscope stage (MS100, Linkam, Surray, UK). 2. Assessment of blastocyst morphology quality is done according to the classification developed by Gardner & Schoolcraft 1999 and re-expansion is assessed following overnight culture.

—

Countries

Belgium

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026