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How dental pulp reacts after treatment: a tissue-level study comparing reversible and irreversible pulpitis in adult teeth

Histopathological and immunohistochemical evaluation of pulpal inflammatory responses following pulpotomy in mature permanent teeth with reversible and irreversible pulpitis

Status
Active, not recruiting
Phases
Unknown
Study type
Interventional
Source
ISRCTN
Registry ID
ISRCTN40490739
Enrollment
60
Registered
2025-05-12
Start date
2022-01-25
Completion date
Unknown
Last updated
2025-09-08

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Treatment reversible and irreversible pulpitis in mature permanent teeth Oral Health

Interventions

The clinical assessment began with a comprehensive evaluation including medical history, chief complaint, pulp sensitivity via cold testing (ethyl chloride), percussion testing for periapical conditio

Sponsors

Damascus University
Lead Sponsor

Eligibility

Sex/Gender
All

Inclusion criteria

Inclusion criteria: 1. A mature permanent tooth indicated for extraction for orthodontic reasons that does not have any carious lesions for the reversible pulpitis groups 2. Mature permanent tooth requiring extraction (such as third molars) has deep carious lesions with clinical signs and symptoms of irreversible pulpitis (spontaneous or intermittent acute pain) for irreversible pulpitis groups 3. A positive response to pulp sensitivity tests with no signs of pulp necrosis (such as edema, fistula or severe sensitivity to percussion) 4. Restorable tooth without the need for a crown or posts, the gingival sulcus depth between 1-3 mm, and normal tooth mobility 5. The periapical radiograph showed a pulp chamber with relatively normal dimensions without degenerative activity (pulp stones, calcification, severe canal narrowing in the vertical direction) 6. Absence of periapical and furcation radiolucency 7. Absence of internal or external absorption

Exclusion criteria

Exclusion criteria: 1. History of immune or bleeding diseases 2. Medications that may affect the metabolism 3. Uncontrolled pulp hemorrhage after pulpotomy procedures within 10 minutes of hemostasis 4. Abnormal color and appearance of the pulp tissue 5. Absence of hemorrhage or little hemorrhage in a dark red color after pulp exposure or after pulpotomy procedures

Design outcomes

Primary

MeasureTime frame
The severity of inflammation was measured using histology after a week and 3 months by examining inflammatory cells, and the severity of inflammation was classified as follows: score 0: There are no inflammatory cells at or adjacent to the site of exposure, score 1: slight inflammation; presence of a small number of inflammatory cells, score 2: moderate inflammation medium number of inflammatory cells, score 3: Acute inflammation; abundant presence of inflammatory cells.

Secondary

MeasureTime frame
The expression of the inflammatory immune marker was measured after a week and 3 months using the amputation materials used according to the following index: score 0: no inflammation (no staining), score 1: mild inflammation (mild staining), score 2: moderate inflammation (moderate staining), score 3: severe inflammation (severe staining)

Countries

Syria

Contacts

Public ContactHasan Alzoubi
dr.hasan.alzoubi.93@gmail.com+963943647659

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026