Impact of glucosamine supplementation on gut health in healthy subjects Not Applicable
Conditions
Interventions
On a daily basis, participants were instructed to ingest 3,000 mg of Glucosamine Hydrochloride (GlucosaGreen® TSI Group Ltd. MT, USA) or a matching placebo (maltodextrin), identical in appearance for
Sponsors
TSI Group
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Between the ages of 18 – 50 years 2. Body mass index between 18.5 – 27 kg/m² 3. Weight stable for the past three months (less than 5% variation in body mass) 4. Free from all cardiovascular, pulmonary, autoimmune, musculoskeletal, gastrointestinal, psychological, or other diseases or disorders, as reported in their medical history
Exclusion criteria
Exclusion criteria: 1. Diagnosed with or were being treated for celiac disease, lactose intolerance, digestive insufficiencies, or other gastrointestinal complications such as irritable bowel syndrome, ulcerative colitis, etc. 2. A current smoker or had quit within the past six months 3. Currently using anabolic steroids or any illicit or recreational drugs 4. Currently taking any antibiotics, probiotics or medication known to impact study outcomes 5. Actively trying to lose weight which included participating in diets that would impact study outcomes (ketogenic or low carbohydrate diet)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Fecal metabolome (short-chain fatty acids, amino acids, amino acid breakdown products, nucleotide breakdown products, microbially produced fermentation products, likely diet-derived products) is measured using stool samples at baseline, after 3 weeks of supplementation, after 3 weeks of washout, after 3 weeks of supplementation 2. Fecal Microbiota is measured using stool samples at baseline, after 3 weeks of supplementation, after 3 weeks of washout, after 3 weeks of supplementation and analyzed for microbiota diversity, bacterial taxa) 3. Stool consistency will be analyzed via the Bristol Stool Chart at baseline, after 3 weeks of supplementation, after 3 weeks of washout, after 3 weeks of supplementation 4. Gut health will be measured via the Gastrointestinal Symptom Rating Scale (GSRS) questionnaire at baseline, after 3 weeks of supplementation, after 3 weeks of washout, after 3 weeks of supplementation | — |
Secondary
| Measure | Time frame |
|---|---|
| Safety measured using adverse event monitoring throughout the study | — |
Countries
United States of America
Outcome results
None listed