Dental caries Oral Health Dental caries
Conditions
Interventions
Twenty-seven volunteers are enrolled in the study, 14 caries active individuals with at least three active dentin caries lesions and 13 caries inactive individuals.
Polished bovine enamel slabs moun
Sponsors
Deutsche Forschungsgemeinschaft
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Test group caries active: 1. Aged between 18 and 45 2. Minimum 24 teeth 3. Written informed consent 4. Recruitment in the outpatient departments of Saarland and Dresden Universities 5. Sufficient oral hygiene (plaque index < 50%, bleeding index < 15%) 6. Three or more active caries lesions (need of invasive treatment) Control group caries inactive: 1. Aged between 18 and 45 2. Minimum 24 teeth 3. Written informed consent 4. Recruitment in the outpatient departments of Saarland and Dresden Universities 5. Sufficient oral hygiene (plaque index < 50%, bleeding index < 15%) 6. No active caries lesions, no new fillings within the last 2 years
Exclusion criteria
Exclusion criteria: 1. Smokers 2. Pregnancy 3. Drug use 4. Periodontal disease 5. Other oral diseases except caries 6. Other diseases and conditions
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Differences in the biofilms are assessed by amplicon sequencing of the V1 and V2 variable regions of the 16S rRNA gene using MiSeq on samples collected at 2, 4 and 8 hours. | — |
Secondary
| Measure | Time frame |
|---|---|
| No secondary outcome measures | — |
Countries
Germany
Outcome results
None listed