Sports nutrition and ergogenic aids Nutritional, Metabolic, Endocrine
Conditions
Interventions
The trial order for the 12 subjects was randomized so that six subjects started with eight weeks CLA trial, while six subjects started with eight weeks placebo trial.
Subjects participated in this cr
Sponsors
National Science Council (Taiwan)
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: For the successful completion of the proposed study, twelve male athletes, aged around 20 years will be recruited from National Taichung University and National Taiwan College of Physical Education. All subjects have been participated in regular exercise training at least three years.
Exclusion criteria
Exclusion criteria: Subjects who have never been accept regular exercise training and are smokers will be excluded to participate this study.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Glycogen assay: approximately 25 mg of skeletal muscle from the deep portion of the vastus lateralis was dissolved in 1 N KOH at 75°C for 30 minutes. Dissolved homogenate was neutralised by glacial acetic acid and incubated overnight in acetate buffer (0.3 M sodium acetate, pH to 4.8) containing amyloglucosidase (Boehringer Mannheim, Indianapolis, IN). The reaction mixture was neutralised with 1 N NaOH. Samples were then analysed by measuring glucosyl units by the Trinder reaction (Sigma, St. Louis, MO). | — |
Secondary
| Measure | Time frame |
|---|---|
| Protein expressions of GLUT4, p-Akt measured with western blotting | — |
Countries
Taiwan
Outcome results
None listed