Spermatogenesis Not Applicable
Conditions
Interventions
Subjects were initially randomized into one of the two groups, each group consisting of 10 volunteers. All of the subjects underwent testicular warming at 43°C in a water bath 10 times, for 30 min eac
Sponsors
Chinese Academy of Sciences (China)
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: 1. Male 2. 22–50 years old 3. Married and having fathered at least one child 4. No plan to father another child 5. Good health without hypertension or trauma
Exclusion criteria
Exclusion criteria: 1. Not married or have no children 2. Plan to have another child 3. Cryptorchidism or varicocele 4. Severe heart, brain or renal disease 5. Could not commit to finishing the experiment
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Semen samples were collected twice before treatment and at week 2, 4, 6, 8, 10, 12, 14 and 16 after treatment. The samples were obtained from each subject by masturbation after between 3 and 7 days of sexual abstinence. 1. Sperm concentration, sperm motility and total sperm count were observed under a microscope. 2. Total acrosin activity was measured using a commercially available kit (HuaKang, Shenzhen, China). 3. Seminal plasma biochemical markers including semen plasma fructose, zinc and neutral alpha glucosidase, were all tested by using enzymic and spectrophotometric methods. 4. Semen plasma oxidative stress was evaluated by the markers of superoxide dismutase (SOD), catalase (CAT) activity and malondialdehyde (MDA), these markers were all tested by using a spectrophotometric method. Venous blood samples were collected before treatment and at week 3, 6, 9, 12, 15 after treatment, 4 ml for each time. Blood samples were used for reproductive hormone assays, including follicle stimulating hormone (FSH), luteinizing hormone (LH), sex hormone binding globulin (SHBG), estradiol (E2), testosterone and free testosterone. All these hormones were tested using a chemiluminescent immunoassay method on the automated UniCel DxI 800 analyzer. | — |
Secondary
| Measure | Time frame |
|---|---|
| Semen samples were collected twice before treatment and at week 2, 4, 6, 8, 10, 12, 14 and 16 after treatment. The samples were obtained from each subject by masturbation after between 3 and 7 days of sexual abstinence. 1. Sperm DNA integrity was detected using flow cytometry 2. Sperm apoptosis was evaluated using mitochondrial membrane potential commercial assay kit and TdT-mediated dUTP nick-end labeling (TUNEL) assay kit 3. Sperm protein levels were analyzed by using a western blotting method. | — |
Countries
China
Outcome results
None listed