Overweight, obesity Nutritional, Metabolic, Endocrine Obesity
Conditions
Interventions
In this study with a crossover design, 10 healthy subjects were studied under temperature controlled conditions after an overnight fast. After the ingestion of capsulated mustard (10 grams) or unpacka
Sponsors
Institute of Metabolic Science
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Healthy volunteers 2. Men and women 3. Non-smokers 4. Age between 17 and 65 years 5. No known medical conditions 6. Not taking any medications or supplements likely to influence energy expenditure
Exclusion criteria
Exclusion criteria: 1. Known medical conditions 2. Using medication or supplements likely to influence energy expenditure or other metabolic parameters
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Energy expenditure (thermogenesis), measured using by indirect calorimetry using a ventilated canopy respiratory gas exchange (GEM; GEMNutrition, Daresbury, UK) at baseline and 30, 60, 90, 120 and 150 minutes after the intervention | — |
Secondary
| Measure | Time frame |
|---|---|
| Measured at baseline and 30, 60, 90, 120 and 150 minutes after the intervention: 1. Core body temperature, measured using a temperature pill (VitalSense, Respironics, Bend, OR, USA) 2. Cold and hunger sensations, determined by asking the participant to rate the sensation of cold of the whole body and hands separately on a 1 to 10 scale, with ratings as following; 1 was rated as not at all cold and 10 was the coldest one had ever felt. Similarly for the degree of hunger, with ratings as: 1 for not hungry at all, and 10 was rated as the most hungry one had ever felt 3. Plasma metabolic parameters; blood samples taken via an indwelling venous catheter 3.1. Glucose measured using the Hexokinase method on a Siemens Dimension RXL AutoAnalyser, reagents and calibrators purchased from Siemens 3.2. Non-esterified free fatty acids (NEFAs) measured using the Roche Free Fatty Acid kit 3.3. Free thyroxin (fT4) measured by time-resolved fluorescence immunoassay on an AutoDELFIA analyser (Perkin Elmer) using kits from Perkin Elmer 3.4. Cortisol measured by fluorescence immunoassay on the Siemens Centaur Autoanalyser 4. Energy intake determined by using the Universal Eating Monitors (UEM)(The Sussex Meal Patterning System) during the test meal at 160 minutes after the intervention. Subjects ate a homogenous test meal (e.g. pasta) containing normal energy percent ratios (~30% carbohydrates, ~30% protein and ~40% fat). Test meal intake continuously monitored using the UEM equipment | — |
Countries
United Kingdom
Outcome results
None listed