Chronic kidney disease Urological and Genital Diseases Chronic kidney disease
Conditions
Interventions
A one-off muscle sample will be collected from the leg of CKD patients and matched controls as well as a blood and urine sample. These samples will be used in laboratory experiments to understand the
Sponsors
University Hospitals of Leicester (UK)
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: CKD patients: 1. Over 18 years of age 2. Diagnosed CKD stage 3b-5 3. If diabetic, good control - HbA1c <9% Matched controls - will be matched to CKD patients for age, sex and race. 1. Over 18 years of age 2. Kidney function < 80mL/min/1.73m2 3. Good general health
Exclusion criteria
Exclusion criteria: CKD patients: 1. Aged under 18 years 2. Uncontrolled diabetes mellitus (HbA1c >9%) 3. Inability to give consent for any reason Matched controls: 1. Aged under 18 years 2. Presence of CKD (eGFR>80mL/min/1.73m2) 3. Presence of other diseases known to cause muscle wasting (some cancers, sepsis, burns and HIV) 4. Inability to give informed consent for any reason.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Muscle fibre size is measured using immunohistochemistry. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Differences in the processes of myogenesis measured using flow cytometry, immunohistochemistry and PCR 2. Differences in protein turnover measured using uptake and release labelling experiments 3. MicroRNA expression and involvement in any dysregulation seen above measured using next generation sequencing and PCR 4. Amino acid transport and intracellular concentrations 5. Muscle architecture 6. Metabolic flexibility and mitochondrial function measured using high resolution respirometry at baseline, 6, 12 and 24 months 7. Differences in physical activity levels between groups determined using patient questionnaires at baseline, 6, 12 and 24 months | — |
Countries
England, United Kingdom
Outcome results
None listed