Diabetic nephropathy Urological and Genital Diseases Diabetic nephropathy
Conditions
Interventions
A total of 134 patients with type 2 diabetes and 20 participants with normal glucose tolerance are enrolled. Patients with type 2 diabetes are divided into three groups based on their urinary albumin
Sponsors
National Natural Science Foundation of China
The New Century Excellent Talentsin University from the Ministry of Education
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Patients: 1. Aged between 20 and 80 years old 2. Diagnosed with type 2 diabetes mellitus Controls: 1. Aged between 32 and 72 years 2. Normal glucose control
Exclusion criteria
Exclusion criteria: 1. Past history of malignancy 2. Degenerative disease of the nervous system 3. Diabetic macrovascular complications 4. Other endocrine diseases which affect glucose metabolism and lipid metabolism 5. Chronic hepatitis 6. Primary kidney disease 7. Recent inflammatory disease 8. Acute trauma 9. Taking thiazolidinedione drugs in the previous 3 weeks 10. Pregnancy 11. History of drug abuse.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. PGRN serum concentration is measured using ELISAs at baseline and 2 hours after overnight fasting 2. Plasma glucose is measured using glucose oxidase method at baseline and 2 hours after overnight fasting 3. Serum insulin is measured using radioimmunoassay at baseline and 2 hours after overnight fasting 4. HbA1c values are measured using high-performance liquid chromatography at baseline and 2 hours after overnight fasting | — |
Secondary
| Measure | Time frame |
|---|---|
| Autophagy indicators Atg7, LC3, and p62 of renal tissue are measured using immunohistochemical examination, real-time PCR and western blotting at baseline, 0, 10, 20 and 40 minutes after overnight fasting. | — |
Countries
Afghanistan
Outcome results
None listed