Digestion in healthy participants Digestive System
Conditions
Interventions
This study consists of one 4-day study visit and three 3-day study visits. At least one week’s gap is needed. The 4-day study visit will mainly look at the upper gastro-intestine (GI) while the 3-day
Guts samples will be taken through a nasoenteric tube to monitor the digestive behaviours and structures breakdown
VAS will be conducted to measure subjective appetite
Urine and stool samples will be collected to measure the metabolites.
Randomisation is conducted using the website of sealed envelope: https://www.sealedenvelope.com/
Sponsors
Imperial College London
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Age between 18 - 65 years (inclusive) 2. Body mass index (BMI) of 18-30 kg/m² 3. Willingness and ability to give written informed consent and willingness and ability to understand, to participate and to comply with the study requirements
Exclusion criteria
Exclusion criteria: 1. Abnormal ECG 2. Screening blood results outside of normal reference values 3. Weight change of = 5kg in the preceding 2 months 4. Current smokers 5. History of substance abuse and/or excess alcohol intake • Pregnancy • Diabetes • Cardiovascular disease 6. Cancer 7. Gastrointestinal disease e.g. inflammatory bowel disease or irritable bowel syndrome 8. Kidney disease 9. Liver disease 10. Pancreatitis 11. Started new medication within the last 3 months likely to interfere with energy metabolism, appetite regulation and hormonal balance, including anti-inflammatory drugs or steroids, antibiotics, androgens, phenytoin, erythromycin or thyroid hormones 12. Participation in a research study in the 12 week period prior to entering this study 13. Any blood donation within the 12 week period prior to entering this study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Appetite-regulating gut hormones measured using RIA assay in the intestinal contents that will be collected through a nasoenteric tube at 0, 60, 120, 180, 240, 300, 420, 480 min at each study day | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Circulating concentrations of glucose, insulin, and metabolites measured using ci8200 analyser enzymatic method, RIA assay, and GC-MS respectively at 0, 60, 120, 180, 240, 300, 420, 480 min at each study day 2. Subjective measures of appetite, as assessed by Visual Analogue Scales (VAS) at 0, 60, 120, 180, 240, 300, 420, 480 min at each study day 3. Energy intake as determined by an ad libitum meal at 240 min at each study day | — |
Countries
England, United Kingdom
Contacts
Public ContactMingzhu Cai
Outcome results
None listed