The effect of a one off bout of cycling exercise on the release of small proteins into the circulation in haemodialysis patients

Exosome release following a single bout of cycling exercise in chronic kidney disease – the ENCODE study

Status

Recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN17464516

Enrollment

Registered

2023-02-16

Start date

2023-03-01

Completion date

Unknown

Last updated

2023-02-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Cardiovascular risk in haemodialysis patients Urological and Genital Diseases

Interventions

Participants will be asked to cycle at a 'somewhat hard' exercise intensity on a static bike. This intensity will be determined during a familiarisation session beforehand and will be re-checked with

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: Patients undergoing maintenance haemodialysis for more than 3 months

Exclusion criteria

Exclusion criteria: 1. Age <18 years 2. Pregnancy 3. Inability to give informed consent 4. Physical disability that prevents exercise 5. Blood borne diseases

Design outcomes

Primary

Measure	Time frame
Characterisation of exosome release following 30 mins of acute cycling exercise in patients with chronic kidney disease, measured by Next Generation Sequencing (raw counts) at baseline, immediately post exercise.	—

Secondary

Measure	Time frame
1. Define the time course of exosome release following acute cycling exercise, measured using the Nanosight (particle numbers) at baseline, immediately post, 10, 30, 60 and 90 minutes post exercise. 2. Characterise the microRNA profiles within circulating exosomes, measured using PCR (cT values) in samples taken at 10, 30, 60 and 90 minutes post exercise. 3. Relate microRNA profiles to clinical data regarding clinical remodelling determined using regression modelling (r2 values) using values immediately post exercise and their baseline characteristics.	—

Measure

Time frame

1. Define the time course of exosome release following acute cycling exercise, measured using the Nanosight (particle numbers) at baseline, immediately post, 10, 30, 60 and 90 minutes post exercise. 2. Characterise the microRNA profiles within circulating exosomes, measured using PCR (cT values) in samples taken at 10, 30, 60 and 90 minutes post exercise. 3. Relate microRNA profiles to clinical data regarding clinical remodelling determined using regression modelling (r2 values) using values immediately post exercise and their baseline characteristics.

—

Countries

England, United Kingdom

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026