Exploration of genetic and environmental factors associated with chronic kidney disease in patients of African ancestry Urological and Genital Diseases
Conditions
Interventions
In the study, plasma, serum, urine, pre-existing renal tissue and peripheral blood mononuclear cells (PBMC) will be collected from patients. These will be sent for further analyses for:
1. Genotyping
Sponsors
King's College Hospital NHS Foundation Trust
King's College London
Eligibility
Sex/Gender
All
Age
18 Years to 100 Years
Inclusion criteria
Inclusion criteria: CKD cases: 1. Self-reported African ancestry 2. Aged =18 years 3. Willing and able to provide written informed consent 4. Chronic kidney disease (KDIGO Criteria) 5. Group 1: Without diabetic nephropathy or serological or biopsy-proven evidence of immune-mediated kidney disease 6. Group 2: With diabetic nephropathy or serological or biopsy-proven evidence of immune-mediated kidney disease Controls: 1. Self-reported African ancestry 2. Aged =18 years 3. Willing and able to provide written informed consent 4. No CKD (estimated GFR >60 ml/min/1.73m² and urinary protein: creatinine <15mg /mol or albumin: creatinine ratio <3 mg/mmol or negative urine dip for protein).
Exclusion criteria
Exclusion criteria: For both CKD cases and controls: 1. Unable to provide informed consent 2. Pregnancy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| At a single time point: 1. APOL1 promoter DNA methylation patterns in renal tissue and PBMC (spleen) and urine (Cases only) measured using standard laboratory analysis 2. CKD disease phenotype measured using standard laboratory analysis | — |
Secondary
| Measure | Time frame |
|---|---|
| At a single time point: Clinical characteristics from patient notes and laboratory records, inflammatory markers measured using novel proteomic methods | — |
Countries
England, United Kingdom
Outcome results
None listed