Biomarkers of food intake Nutritional, Metabolic, Endocrine
Conditions
Interventions
Volunteers will consume one of the following foods in a random order: lentils, chickpeas, white beans and pasta. These foods will be consumed on separate study visits, and baseline and postpandrial bl
Sponsors
University of Barcelona
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Healthy adults aged between 18 and 40 years old 2. Non-smokers 3. BMI >18.5 or 55 kg 5. Free of chronic or infectious disease 6. Not pregnant, planning a pregnancy or lactating 7. Not taking any regular medication (oral contraceptive pill is allowed) 8. Not taking supplements 9. No antibiotics in the 6 previous months 10. No allergy or aversion to legumes or pasta 11. Not anaemic 12. No blood donation within the last 2 months
Exclusion criteria
Exclusion criteria: 1. Smokers 2. Diagnosed anaemia or another health condition (chronic or infectious disease) 3. Blood donation during the previous 2 months 4. Allergy, intolerance or aversion to legumes or pasta 5. Taking medication or supplements (oral contraceptive pill is allowed) 6. Pregnant, planning a pregnancy or lactating
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Presence of biomarkers of legumes consumption in blood and urine, measured using an untargeted metabolomics approach. Fasting blood and urine samples are collected before the intake of each food. Then, postpandrial blood samples are taken after 1, 2, 4 and 6h; and additional fasting blood samples 24 and 48 hours after the intake of each food. In parallel, urine samples are also collected in the following intervals post-consumption of each food: 0-1h, 1-2h, 2-4h, 4-6h, 6-12h, 12-24h, 24-48h. | — |
Secondary
| Measure | Time frame |
|---|---|
| No secondary outcome measures | — |
Countries
Spain
Outcome results
None listed