Effect on dental pulp of different methods of cavity preparation

Histological evaluation of the human pulp response to different cavity instrumentation and restorative techniques

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN17017801

Enrollment

Registered

2018-09-21

Start date

2008-02-01

Completion date

Unknown

Last updated

2019-12-10

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Dental pulp injury following cavity preparation Oral Health

Interventions

Participants were allocated in 5 experimental groups. Groups 1 to 4 received local anesthesia with adrenaline (4% Articaine), rubber dam isolation and deep class V cavities as follows:

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: 1. Sound teeth without signs of cervical lesion 2. Sound teeth without signs of cracks or fractures 3. Sound teeth without signs of periodontal disease 4. Sound teeth without signs of root lacerations 5. Sound teeth with normal response to cold (fast decrease of sensitivity after cold stimuli) 6. Sound teeth without sensitivity to percussion. 7. Patient aged 11-25 years

Exclusion criteria

Exclusion criteria: 1. Teeth with existing caries lesions 2. Teeth with periodontal disease 3. Teeth with cervical lesions

Design outcomes

Primary

Measure	Time frame
Morphological features of the pulp tissue. The teeth were extracted according to the postoperative experimental periods, immediately, 7 and 30 days after the cavity preparation under local anesthesia and fixed in 10% phosphate-buffered formalin solution for 48 hours. subsequently, the teeth were decalcified in 4% EDTA (Titriplex III, Merck) solution (pH 7.4) and submitted to histological procedures. Five-micrometer thick serial sections were cut parallel to the main vertical axis of the tooth throughout the cavity preparation, stained with hematoxylin and eosin (H&E) and Brown and Brenn (B&B) and analyzed under an Olympus BX50F4 light microscope. Each series of tooth sections were blind analyzed by one experienced examiner and the data of all morphological features of the pulp tissue were fully recorded.	—

Measure

Time frame

Morphological features of the pulp tissue. The teeth were extracted according to the postoperative experimental periods, immediately, 7 and 30 days after the cavity preparation under local anesthesia and fixed in 10% phosphate-buffered formalin solution for 48 hours. subsequently, the teeth were decalcified in 4% EDTA (Titriplex III, Merck) solution (pH 7.4) and submitted to histological procedures. Five-micrometer thick serial sections were cut parallel to the main vertical axis of the tooth throughout the cavity preparation, stained with hematoxylin and eosin (H&E) and Brown and Brenn (B&B) and analyzed under an Olympus BX50F4 light microscope. Each series of tooth sections were blind analyzed by one experienced examiner and the data of all morphological features of the pulp tissue were fully recorded.

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Secondary

Measure	Time frame
1. The volume densities or the fractions of pulp volume occupied by the odontoblasts, collagen fibers and fibroblasts, ground substance, blood vessels, inflammatory process and tertiary dentin adjacent to the cavity preparation was performed with the point-counting relative volumetry method using a 100× immersion objective and a Zeiss Kpl 8× eyepiece containing a Zeiss II integration grid (Carl Zeiss GmbH, Jena, Germany) with 100 points in a quadrangular area. In ten sections per tooth, the grid was successively superimposed on 12 histological fields per section at three different pulp levels. The points that were superimposed on each cell or structure (Pi) were counted and the total number of points (Pt) was obtained. Volume density (Vvi) was calculated by the equation: Vvi = Pi/Pt. 2. Remaining dentine thickness (RDT). For each tooth, five histological sections with less RDT were selected. Images of the remaining dentin were captured in a video camera AxioCam HRc (Carl Zeiss MicroImaging GmbH, Göttingen, Germany) coupled to an Axioskop II microscope (Carl Zeiss, Oberköchen, Germany) with 10× objective and analyzed using the AxioVision Release 4.7.2 software (Carl Zeiss Vision Imaging Systems, Oberköchen, Germany). The RDT of each group was the average of the RDT of each specimen.	—

Measure

Time frame

1. The volume densities or the fractions of pulp volume occupied by the odontoblasts, collagen fibers and fibroblasts, ground substance, blood vessels, inflammatory process and tertiary dentin adjacent to the cavity preparation was performed with the point-counting relative volumetry method using a 100× immersion objective and a Zeiss Kpl 8× eyepiece containing a Zeiss II integration grid (Carl Zeiss GmbH, Jena, Germany) with 100 points in a quadrangular area. In ten sections per tooth, the grid was successively superimposed on 12 histological fields per section at three different pulp levels. The points that were superimposed on each cell or structure (Pi) were counted and the total number of points (Pt) was obtained. Volume density (Vvi) was calculated by the equation: Vvi = Pi/Pt. 2. Remaining dentine thickness (RDT). For each tooth, five histological sections with less RDT were selected. Images of the remaining dentin were captured in a video camera AxioCam HRc (Carl Zeiss MicroImaging GmbH, Göttingen, Germany) coupled to an Axioskop II microscope (Carl Zeiss, Oberköchen, Germany) with 10× objective and analyzed using the AxioVision Release 4.7.2 software (Carl Zeiss Vision Imaging Systems, Oberköchen, Germany). The RDT of each group was the average of the RDT of each specimen.

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Countries

Brazil

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026