Dermatology Skin and Connective Tissue Diseases
Conditions
Interventions
Previous laboratory research has shown that skin bacteria are able to influence the response of skin cells to UVR, and that cleaning forearm skin with 70% ethanol (as commonly found in anti-bacterial
Sponsors
University of Manchester
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Healthy adults of any gender aged 18-40 years 2. White Caucasian (Fitzpatrick skin type I-III) 3. Able to understand and meet study requirements, and provide written informed consent
Exclusion criteria
Exclusion criteria: 1. Skin sensitivity/allergy to alcohol-based skin preparations 2. Existing significant (moderate-severe) skin disease such as eczema, xerosis (dry skin), psoriasis 3. Use of antimicrobial medications in the last 3 months 4. Sunbathing/sunbed use/phototherapy in the last 3 months 5. Currently participating, or have recently participated (within the last month) in other research 6. Unable to meet the requirements for participating in the study Additional exclusions for the main study: 1. History of photosensitivity disorder or skin cancer 2. History of bleeding disorders 3. Taking photoactive, anti-inflammatory or anti-coagulant medication
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| UVR-induced erythema assessed visually (minimal erythema dose) and objectively by spectrophotometry at 24 hours | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Markers of inflammation, DNA damage, apoptosis, and cell proliferation measured by immunofluorescent staining at baseline and 24 hours 2. Density and diversity of skin microbiota measured by 16S rRNA sequencing at baseline and 24 hours | — |
Countries
England, United Kingdom
Outcome results
None listed