Relevance of bovine milk exosomes as bioactive food compounds and use of exosomes for drug delivery. Other
Conditions
Interventions
12 healthy adults received 5 different milk meals in a randomized cross-over design, with a washout period of at least 1 week between each meal. The milk meals were 1 l of 1% fat bovine milk, 1 l of 1
Sponsors
The Gerber Foundation
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Healthy adults
Exclusion criteria
Exclusion criteria: 1. Pregnant women 2. Smokers 3. Persons with lactose and milk protein intolerance or gastrointestinal disorders
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Levels of six immune-relevant microRNA found in bovine milk exosomes in plasma using real-time quantitative polymerase chain reaction (RT-qPCR) before (0 h) and at timed intervals (3, 6,and 9 h) after a milk meal | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Secretion of inflammation-related cytokines by cultured human peripheral blood mononuclear cells (PBMCs) collected before and 6 h after milk consumption and stimulated with or without Concanavalin A (Con A) was assessed using a customized Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panel Immunoassay 2. Secretion of cytokines by PBMCs treated ex vivo with microRNA-loaded exosomes assessed using a customized Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panel Immunoassay | — |
Countries
United States of America
Contacts
Public ContactEzra Mutai
Outcome results
None listed