Aetiology of sarcopenia in patients with chronic kidney disease Urological and Genital Diseases
Conditions
Interventions
Participation will involve a single research visit for sharing information on health and lifestyle, testing muscle function, and donating blood, urine, and muscle tissue samples. Samples will then be
Sponsors
University of Birmingham
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Men and women 2. Age 60-80 years 3. For CKD group (recruitment target 30): chronic kidney disease stage IV or V (eGFR less than 30ml/min for more than 3 months) 4. For control group (recruitment target 20): no history of kidney disease or other major chronic illness 5. Willing and able to provide informed consent
Exclusion criteria
Exclusion criteria: 1. Receive steroid medications or hormone replacement therapy other than thyroxine for well-controlled hypothyroidism 2. Have a primary neurological or muscle disease that impairs muscle function 3. Be at extremes of body weight (BMI 35kg/m²) 4. Have a diagnosis of cancer in the past 5 years (except non-melanoma skin cancer) 5. Suffered an acute illness requiring hospital admission within the past month 6. Have a bleeding predisposition or receive anticoagulation (except aspirin that can be suspended for 3 days without significant clinical risk) 7. Be enrolled in another clinical trial with a treatment intervention within past four months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Conversion rate of cortisone to cortisol measured in skeletal muscle biopsies ex vivo at baseline using a radiolabelled tracer assay. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Urinary steroid metabolome measured using liquid-chromatography/mass-spectrometry at baseline 2. Handgrip strength measured using a handheld dynamometer at baseline 3. Body composition measured using bioelectrical impedance analysis at baseline 4. Physical function measured using the Short Physical Performance Battery test at baseline 5. Malnutrition measured using the Short Nutrition Assessment Questionnaire at baseline 6. Physical activity measured using the International Physical Activity Questionnaire (short form) at baseline) 7. Serum cytokine and hormone levels measured by ELISA and LUMINEX assays at baseline 8. Expression of anabolic and catabolic markers in skeletal muscle biopsies using qPCR and Western blot analysis at baseline | — |
Countries
England, United Kingdom
Outcome results
None listed